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Figure 5.
FIGURE 5. Binding of the human wild-type and D145N/H268N
UNG enzymes to non-cleavable substrate analogs d  rd and
 -FdUrd. The binding of
hexachlorofluorescein-labeled oligonucleotides containing the
non-cleavable substrate analogs d rd and -FdUrd
was monitored using fluorescence polarization. A, the binding of
wild-type hUNG was measured with d rd (•) and -FdUrd (
 ).
Data are shown with the best fit to the binding equation with
the following values: d rd, K[d] = 4.4 ±
0.5 µM, A[D] = 0.041 ± 0.002, and A[D][E] = 0.17
± 0.003; and -FdUrd, K[d] = 6.3
± 0.6 µM, A[D] = 0.039 ± 0.002, and A[D][E]
= 0.13 ± 0.002. B, the binding of human D145N/H268N UNG
was measured with d rd (•) and -FdUrd (
).
Data are shown with the best fit to the binding equation with
the following values: d rd, K[d] = 3.2 ±
0.2 µM, A[D] = 0.038 ± 0.001, and A[D][E] = 0.14
± 0.001; and -FdUrd, K[d] = 2.2
± 0.2 µM, A[D] = 0.041 ± 0.003, and A[D][E]
= 0.17 ± 0.002.
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