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Figure 5.
Figure 5. Structural "snapshots" of the PSP reaction cycle
in the active site. (a) The PSP reaction cycle. (b) Structure
and models in the active site. (I) The apo-enzyme structure.
(II) The substrate, PLS, bound structure, using mutant D11N.
(III) The model of Ser bound transition state structural
analogue. A Ser molecule was modeled in the AlF[3]-PSP active
site. (IV) Phospho-aspartyl enzyme intermediate structural
analogue. The PSP+BeF[3]^ - complex. (V) The transition state
structural analogue. AlF[3]-PSP complex. (VI) The product, Pi,
bound structure.
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