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Figure 5.
Fig. 5. Oxidation of OYE1 by 2-cyclohexenone. OYE1 was
reduced by an NADPH-generating system consisting of
glucose-6-phosphate (0.625 mM), glucose-6-phosphate
dehydrogenase (5 µl), and NADP (0.625 µM). Reduction
of the enzyme was followed spectrally and was complete at 84
min. The reduced enzyme was mixed in the stopped flow apparatus
with the concentrations of cyclohexenone shown, and the reaction
traces at 460 nm were used to calculate the values of k[obs]
shown.
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