|
Figure 4.
FIGURE 4. Substrate binding and reaction mechanism of PuuE.
A, surface drawing of hydantoin-bound PuuE (left panel) compared
with a zinc-bound chitin deacetylase (Protein Data Bank code
2IW0; right panel). The inserted segments IS1 and IS2 of PuuE
are colored orange and light blue, respectively. The positions
of sugar-binding subsites in 2IW0 are indicated. B, stereo view
comparison of the metal-binding site of 2IW0 (cyan) and the
corresponding region of PuuE (yellow). Metal-binding residues of
2IW0 and superimposable residues of PuuE are shown as sticks. C,
the PuuE active site in complex with hydantoin. Key residues and
ligands are represented in stick mode. D, proposed catalytic
mechanism for (S)-allantoin hydrolysis.
|
