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Figure 4.
Figure 4. Construction of the Structure of the ThkA/TrrA
Complex
(A) Electron density (final 2F[o] − F[c] map) of
the 2:2 complex at 3.8 Å resolution. Red spheres are the
anomalous differences Fourier maps (3.5 σ) of either Hg or Se
atoms obtained from the crystal of Hg-SeMet-ThkA
(F486M,F489M,H546M)/SeMet-TrrA(D52M,L89M) (see Table S5).
(B) The map (A) is viewed along a two-fold axis and the map is
represented as a grid.
(C) The overall ThkA/TrrA complex in
the 2:2 dimer. The view is the same as in (A).
(D) The
structure viewed along a two-fold axis.
(E–H) Qualities
of the fitting into the map (2F[o] − F[c] map contoured at 1
σ) for the PAS domain of ThkA (E), DHp of ThkA (F), CA of ThkA
(G), and TrrA (H).
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