|
Figure 4.
Figure 4. (a) Experimental X-ray scattering pattern from
filamin C Ig-like domains 23–24 dimer. Scattering from a
typical ab initio model computed by DAMMIN (continuous line,
red) and from the three-dimensional model with added linker
parts between domains 23 and 24 obtained by BUNCH^8 (broken
line, blue). The plot displays the logarithm of the scattering
intensity as a function of momentum transfer s = 4π sin(θ)/λ,
where 2θ is the scattering angle and λ = 0.15 nm is the X-ray
wavelength. The distance distribution function is displayed in
the inner window on top right. (b) Ab initio and rigid models of
the filamin C Ig-like domains 23–24 dimer. Ab initio models of
filamin C Ig-like domain 23–24 dimer superimposed with the
rigid body models. The ab initio models obtained by DAMMIN are
displayed as gray semitransparent spheres, the models obtained
by BUNCH as C^α traces (the two monomers are coloured red and
blue, the linkers are green). Top panel, models without
symmetry; bottom panel, models with P2 symmetry axis (vertical
axis on the bottom left). The right view is rotated clockwise
around the horizontal axis. The bar represents the length of 50
Å and is shown at the top left.
|
