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Figure 4.
Figure 4. The Topology of TSPN-1
The topology of (A)
TSPN-1 is compared with that of (B) CTSP-1 (the C-terminal
domain of TSP-1) (Kvansakul et al., 2004) and (C) a typical LG
(Laminin A G-domain [LG]/Neurexin/Sex hormone binding globulin
[SHBG]) repeat (Rudenko et al., 2001). For the LG structure,
only the common positions of the b strands are shown. The
disulfide bonds are highlighted in yellow. Only b strands (cyan)
are labeled for comparison. The a helices of TSPN-1 and CTSP-1
are shown in green. The two structures can't be simply
superimposed. The best alignment can only be made with the
middle strands of the back sheet, b14, b5, b10, and b11 of
TSPN-1 and b15, b5, b11, and b12 of CTSP-1. Even in this
alignment, the b strands from the two front sheets shift over
about one strand and also rotate slightly. The edge strands,
especially the N-terminal edge strands, are totally out of
alignment. The front concaved sheet of CTSP-1 is distinctly
curved, while that of TSPN-1 is relatively flat.
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