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Figure 4.
Fig. 4. Dynamic alterations in the loop[H28-H29] upon
breakage of the disulfide bond. (A–C) The loop[H28-H29] and
the proximal regions in C-PMT (A), C-PMT[1159S] (B), and
C-PMT[1165S] (C) are shown. A  [A]-weighted omit
electron density map (contoured at 3 ) is superimposed on
the stick model of key residues, Cys^1159 (Ser^1159 in B),
Cys^1165 (Ser^1165 in C), His^1205, Asp^1220, and Gln^1225. (D)
Comparison of the active sites among C-PMT[1159S] (light green)
and C-PMT[1165S] (light blue) with papain (pink), AvrPphB (lime
green), and N-acetyltransferase (orange). His^1205, Asp^1220,
and Cys^1165 of C-PMT[1159S] were superimposed on the
corresponding residues of the counterpart proteins by Lsqkab in
the CCP4 program suite (30).
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