|
Figure 4.
FIG. 4. Interactions of H12 with the coactivator groove in
the ER  LBD·4-OHT and
the ERR  LBD·4-OHT
complex (crystal form 1). A, fitting of the fortuitously
co-crystallized cholic acid molecule into a 2F[o] - F[c]
electron density map. B, superimposition of selected parts of
the ERR LBD (orange) and the ER
LBD
(blue) bound to 4-OHT. In the ER LBD·4-OHT
complex, H12 packs against the coactivator cleft of the
respective LBD body with Leu-536 and Leu-540, mimicking LXXLL
coactivator interactions. In the ERR LBD·4-OHT
complex (subunit B in crystal form 1), H12 interacts with the
coactivator groove of a neighboring molecule. Binding is
distinct from that in ER due to the absence of a
LXXLL motif in H12 of ERR . The alternative
packing interactions between H12 and the coactivator groove,
rather, are determined by the fortuitously co-crystallized
cholic acid molecule that occupies part of the cleft.
|
