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Figure 4.
Fig. 4. Overlapping ^1H–^15N HSQC spectra of PRL-3
obtained in the presence and absence of ligand ions. The peaks
representing the free form of the protein are shown in black.
(A) 0.7 mM PRL-3 with 2 mM sodium orthovanadate (brown), (B) 0.7
mM PRL-3 with 20 mM phosphate (purple). The red circled peaks
are the backbone amide protons that appeared in the active site
(phosphate loop).
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