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Figure 4.
Figure 4. In Vitro Interaction Between Wild-Type or Mutant
LBDs of ERR3 and the RID of SRC-1Two micrograms (about 70 pmol)
of partially purified His-tagged ERR3 LBD was preincubated for
10 min on ice with 4 μg (about 100 pmol) of GST-tagged SRC-1
RID or GST in the absence (A) or in the presence ([B] and [C])
of 10^−4 M 4-OHT or 10^−4 M DES. Complexes were separated on
native polyacrylamide gradient gels.
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