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Figure 4.
Fig. 4. Amino acid type at position 95 of Cdc42 or Rac1
is an important determinant for GrafGAP activity. The relative
sensitivity of 5 µM Rho GTPases to activation were
measured in the absence ( open bars) or presence of 50 nM
GrafGAP (dashed bars) or 200 nM GrafGAP (double-dashed bars) by
the nitrocellulose filter binding assay.
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