|
Figure 3.
Suggested mechanisms for the formation of the PAmCherry1 dark
chromophore (OFF state) and its light-induced conversion into
the fluorescent state (ON state) are shown. The cyclized form is
the chromophore with the non-oxidized bond between
p-hydroxyphenyl and imidazolone moieties and without
N-acylimine. Hydrogen bonds are shown with dashed lines.
Intermediate compounds are shown in parentheses. The chromophore
in the excited state is denoted with asterisk. hv, indicates the
illumination with violet light. Migration of the electron
density is shown with curved arrows.
|
