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Figure 3.
Figure 3. Overall Structure of the N-Terminal Domain of BUB1
(A) The two molecules observed in the crystal asymmetric
unit associate to form a dimer with noncrystallographic two-fold
symmetry.
(B) The structure viewed 90° rotated along
the minor axis.
(C) Electron density for α helices H5, H6,
H7, and H9 and the connecting loop (after density modification)
contoured at 1.4 σ. The final, refined model is shown using a
ball-and-stick representation. The α helices, loops, and side
chains are clearly visible in the initial map. Water molecules
are shown as red spheres.
(D) Ribbon diagram showing that
this domain consists of ten α helices with a core arrangement
of a triple repeat of the TPR motif (TPR1 orange; TPR2 magenta;
TPR3 cyan).
(E,F) Superposition of the three TPRs of
Sc-BUB1[(29-230)]. Each molecule representation was generated
with Pymol (DeLano, 2002).
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