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Figure 3.
Figure 3: Interactions of the GGQ region of RF1 in the PTC.
a, Stereo view of  [A]-weighted
3F[obs]–2F[calc] electron density for RF1 (yellow), P-site
tRNA (orange) and 23S rRNA (grey) contoured at 1.7 .
b, Position of Gln 230. c, Model for product stabilization by
hydrogen bonding between the main-chain amide of Gln 230 and the
3'-OH of A76 of the P-site tRNA. d, Superposition of a
peptidyl-transferase transition-state analogue (TSA, orange)
complexed with the 50S subunit (grey)^25 on the structure of the
termination complex (this work). The main-chain amide of Gln 230
is positioned to hydrogen bond with the oxyanion of the TSA. e,
Model for transition-state stabilization.
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