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Figure 3.
(a) Ribbon and stick representation of the complex containing
one MBNL1 ZnF3/4 domain (molecule A) and three RNAs bound by
ZnF3 and ZnF4. The ZnF3/4 molecule A is colored light blue.
Cysteine and histidine residues coordinating zinc atoms are
shown in ball-and-stick representation. The two symmetry-related
RNA strands are colored pink and the third RNA strand is colored
light orange. The G5 and 5'-C1 nucleotides originating from two
RNA strands are bound to ZnF3, whereas the G2-C3-U4 segment of
the third strand is bound to the ZnF4 motif. (b) An
electrostatic view of the molecule A complex generated using the
GRASP and PyMol programs. Basic and acidic regions appear in
blue and red, with the intensity of the color proportional to
the local potential. (c) View of the protein-RNA interface
highlighting intermolecular contacts between G2-C3-U4 segment
and MBNL1 ZnF4. Stacking interactions of G2 and C3 bases with
arginine and aromatic residues, as well as hydrogen-bonding
contacts involving G2, C3 and U4, are highlighted. (d)
Electrostatic surface representation of the protein-RNA
interface in the same view as in c.
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