Figure 3.
Figure 3: Auto-cleaving mechanism of EscU. a, Non-cleaved
type I -bend
of P263A (left), the non-cleaved type II beta bend of N262A
(middle left), the non-cleaved type II beta bend of N262Di
(middle right) and the native cleaved loop with a flipped His
265 (right). Note the identical conformations of the N, C, C
and
C at
position 262 in all uncleaved forms. All maps are sigma-A
weighted 2F[o] - F[c] electron density (1.5 );
water molecules are represented by the red spheres. b, Detailed
mechanism for the asparagine cyclization in EscU.
-bend
of P263A (left), the non-cleaved type II beta bend of N262A
(middle left), the non-cleaved type II beta bend of N262Di
(middle right) and the native cleaved loop with a flipped His
265 (right). Note the identical conformations of the N, C, C
and
C 
);
water molecules are represented by the red spheres. b, Detailed
mechanism for the asparagine cyclization in EscU.