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Figure 3.
Figure 3. Radiation disruption of the active-site disulfide
in HvTrxh2. Stereo images of HvTrxh2[OX] (A) and HvTrxh2[RED2],
80% reduced (B), showing a close-up view of segment
Cys46–Cys49. The 2F [o]–F [c] electron density maps are
shown as gray isosurface mesh at the 1.0  level. (C)
HvTrxh2[RED1] with the 1/V  | F(h)[RED1]
– F(h)[RED2] | e^i ^ RED2(h) e ^–2
 i(h – r)
difference electron density contoured at the 7 level.
Significant negative (red) density represents areas where the
HvTrxh2[RED2] structure has a higher level of electron density
than the HvTrxh2[RED1] structure, and positive (green) electron
density, the opposite. For HvTrxh2[RED1] and HvTrxh2[RED2]
structures, Cys46 and Cys49 are modeled and shown in two
alternative conformations (for oxidized and reduced states).
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