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Figure 3.
(a) F[o] – F[o] difference electron density map, truncated
at 6-Å resolution, comparing 70S ribosome crystals in
complex with RRF to ribosomes in complex with neomycin. In the
absence of RRF, the overall position of H69 at the interface in
apo–70S ribosomes is essentially identical to that in
neomycin-bound ribosomes (Supplementary Methods). Only domain I
of RRF is visible; domain II is located to the right of the view
shown. Blue, positive difference density; red, negative
difference density; arrow, direction of the conformational
change in H69 upon RRF binding. (b) F[o] – F[o] difference
electron density as in a, but at 3.5-Å resolution. Insets
in a and b show angles of view.
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