Figure 3 - full size

Figure 3. FIGURE 3. NMR structures of the DNA-binding domain and chemical shift mapping of the residues important for DNA binding. A, stereoview of the backbone traces from the final ensemble of 20 solution structures. The -helices are displayed in orange and -sheets in blue. B, electrostatic potential surface of the transactivation domain is displayed. Red, blue, and white colors represent negative, positive, and neutral electrostatic potential, respectively. The backside view of the molecule rotated by 180° around the vertical axis is also shown. Residues important for DNA binding are labeled. C, chemical shift change of the effector domain upon DNA binding. The chemical shift changes are calculated by using the equation: [tot] = (( [HN]W[HN])^2 + ( [N]W[N])^2)^1/2, where [i] is the chemical shift of nucleus i, and W[i] denotes its weight factor (W[HN] = 1, W[N] = 0.2). D, residues that exhibit significant chemical shift perturbation upon DNA binding. Magenta indicates [tot] > 0.2, and yellow indicates 0.1 < [tot] <0.2.