Figure 3.
Fig. 3. Solution structure of the CAP-Gly-2–peptide
complex. (a) A best-fit superimposition of the final 20
simulated annealing structures of the CLIP-170 CAP-Gly-2 domain
(residues 212–281) bound to the 3-tubulin peptide
(447–451) with the lowest energies is displayed. The CAP-Gly-2
domain backbone (cyan) and selected side chains (blue) are
depicted with the peptide (magenta). (b) Close-up view of the
hydrogen bonds (dotted lines) formed in the complex along the
arrow in a (Right). The side chain of Phe-236 is omitted for
clarity. (c) Pull-down assay of mutated CAP-Gly-2 domains with
the 3-tubulin peptide
suggests a critical role for Asn-253 in peptide binding.
3-tubulin peptide
(447–451) with the lowest energies is displayed. The CAP-Gly-2
domain backbone (cyan) and selected side chains (blue) are
depicted with the peptide (magenta). (b) Close-up view of the
hydrogen bonds (dotted lines) formed in the complex along the
arrow in a (Right). The side chain of Phe-236 is omitted for
clarity. (c) Pull-down assay of mutated CAP-Gly-2 domains with
the