Figure 3.
FIG. 3. A, the SpnHL binding site for L-ascorbic acid
6-hexadecanoate. Hydrogen bonds are represented as dotted lines.
Shown is the final sigmaA-weighted omit
map electron density calculated to a resolution of 1.65 Å
when the inhibitor was excluded from the model and contoured at
2.0 . B, schematic diagram
of interactions of L-ascorbic acid 6-hexadecanoate with SpnHL.
Hydrogen bonds are represented as dotted lines. Other residues
shown form hydrophobic interactions with the inhibitor. The
figure was made with LIGPLOT (35). C, binding mode of
hexasaccharide substrate. The coordinates of the hexasaccharide
substrate originate from its complex with SpnHL (Protein Data
Bank code 1loh [PDB]
(54)). D, binding mode of L-ascorbic acid 6-hexadecanoate. The
cryoprotectant xylitol molecule close to the inhibitor (see
text) is also shown (bottom right corner).
omit
map electron density calculated to a resolution of 1.65 Å
when the inhibitor was excluded from the model and contoured at
2.0 
. B, schematic diagram
of interactions of L-ascorbic acid 6-hexadecanoate with SpnHL.
Hydrogen bonds are represented as dotted lines. Other residues
shown form hydrophobic interactions with the inhibitor. The
figure was made with LIGPLOT (35). C, binding mode of
hexasaccharide substrate. The coordinates of the hexasaccharide
substrate originate from its complex with SpnHL (Protein Data
Bank code 1loh