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Figure 4.
Figure 4. Estimation of Molecular WeightThe apparent
molecular weight of each indicated complex was analyzed with a
HiLoad 26/60 Superdex 75 column. The proteins were eluted at a
rate of 1.5 ml/min with 30 mM TrisHCl buffer (pH 8.0) containing
100 mM NaCl and 3 mM dithiothreitol. For clarity, the elution
profiles for only CAD:ICAD and CAD are shown. The size marker
proteins were the TRAIL:DR5 complex (99 kDa), albumin (66 kDa),
ovalbumin (45 kDa), chymotrypsinogen (25 kDa), and ribonuclease
A (14 kDa). The calculated molecular weights of CAD, ICAD-L,
ICAD-S, I1, I1-I2, and I2-I3 that we generated are 37.53, 34.42,
28.77, 12.87, 24.76, and 21.56 kDa, respectively.
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