Figure 3 - full size

Figure 3.
FIG. 3. Kinetic mechanism of inhibition of FBPase by OC252. A, data are taken at 1-20 µM F16P[2] in 50 mM Hepes, pH 7.5, with 5 mM MgCl[2]. Curves are fits to the data using Equation 3. B, data are taken at 0.1-5 mM Mg2+ (corrected for chelation by EDTA) in 50 mM Hepes, pH 7.5, with 20 µM F16P[2]. Curves are fits to the data using Equation 4.