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Figure 3.
Figure 3. Fz7 and Dpr/Frodo Peptides and Their Binding to
the PDZ Domain In Vitro(A) Biotinylated Fz7 or Dpr/Frodo peptide
was coupled to the UltraLink Immobilized Monomeric Avidin, and
the avidin-coupled peptides were then incubated with purified
PDZ domain. After extensive washing, peptide-interacting
proteins were resolved and visualized by SDS-PAGE. Besides those
bound to the PDZ domain, some proteins that became detached from
the avidin-immobilized beads were also observed. Lane 1, marker.
Lane 2, PDZ domain. Lane 3, The PDZ domain and the
avidin-agarose beads. Lane 4, The PDZ domain and beads coupled
to the Fz7 peptide. Lane 5, the PDZ domain and beads coupled to
the Dpr/Frodo peptide. Lane 6: the PDZ domain bound to the Fz7
peptide-coupled beads after elution by the Dpr/Frodo peptide.
The experimental condition was similar to that used for the
mixture depicted in lane 4, except that before SDS-PAGE, the
beads were washed with a buffer that contained Dpr/Frodo peptide.
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