Figure 3 - full size

Figure 3. Figure 3 Chemical shift mapping of the PTAP binding site on Tsg101 UEV. (A) Overlay of the ^1H/^15N-HSQC spectra of Tsg101 UEV in the absence (gray) or presence of the 1.0 molar equivalent of PTAP peptide (red). The lower panels show an expansion of the boxed region with an additional titration point indicating that the Tsg101 UEV/PTAP complex is in slow exchange. (B) Biosensor binding of Tsg101 UEV to immobilized full-length p6 (upper left panel and closed circles) and PTAP peptide to immobilized Tsg101 UEV (lower left panel and open circles). The near superimposition of the two curves demonstrates that Tsg101 UEV binds p6 and the PTAP peptide with the same affinity.