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Figure 3.
Fig. 3. Mapping of functional data onto the structure of
CD55[34]. This figure was drawn using AESOP (M. E. M. Noble,
unpublished program). All plates show two views of the surface
of CD55, the first view corresponding to the orientation as
shown in Fig. 1 and termed Front View. The Back View corresponds
to a rotation of 180 degrees about the long axis of the
molecule. A, orange indicates the sites mutagenized in this
study, and yellow indicates the sites of sequence difference
between AGM and human CD55. B, AP indicates the mutation shown
to effect alternative pathway decay acceleration, and CP
indicates those sites shown to affect classical pathway decay
acceleration; both those sites have been shown to affect both
pathways. Dark green indicates those sites shown previously to
effect both classical and alternative pathway decay acceleration
(22). C, sites marked with a virus name have been shown to
affect binding of that virus. Sites marked in pale pink are the
sites of difference between AGM and human CD55 that are known to
abolish EV11 binding and significantly reduce EV12 binding. D,
the locations of two of the Cromer variants of human CD55 are
shown. Different E. coli strains are sensitive to changes at one
or other of these positions (28, 30, 32).
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