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Figure 2.
Residue 148 of RseA plays an important role for Site-2
cleavage. (A) Mutation of Val-148 to Thr or Ile, but not His or
Lys, allowed Site-2 cleavage. DegS and OMP peptide were added
together to the reactions where DegS is indicated. (B) Mutation
of Val-148 to Thr or Ile in RseA allowed a more robust envelope
stress response than mutation of Val-148 to His or Lys.
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