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Figure 2.
Figure 2. Structure-Based Sequence Alignment of PTGR2 and
Homologs
(A) Stereo view of the crystal structure of the
hPTGR2-NADP^+-15-keto-PGE[2] complex. The nucleotide-binding
domain (H149–Y292) and catalytic domain (M1-G148; K293–L351)
are colored in purple and orange, respectively. The NADP^+
(yellow carbons), 15-keto-PGE[2] (green carbons), and sulfate
ions (dark yellow sulfurs) are shown as ball-and-stick models.
(B) Multiple sequence alignments of human PTGR2 (SwissProt
entry Q8N8N7), mouse PTGR2 (Q3TG36), guinea pig 13-PGR/LTB[4]DH
(Q9EQZ5), and human 13-PGR/LTB[4]DH (A8K0N2). The LID motifs and
PPII helices of PTGR2 are indicated by double-headed arrows. The
conserved nucleotide-binding motif is underlined. Tyr64 and
Tyr259 are marked with red boxes.
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