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Figure 2.
Figure 2. Structure of Myristoylated yARF1 and Comparisons to
Related Structures
(A) Overlap of 15 accepted
myr(+)-yARF1·GDP structures out of 100 trials. GDP is
shown in blue and myristoyl is in purple.
(B) The myristoyl
binding pocket with leucines shown in red, isoleucines in blue,
tyrosines in cyan, and myristoyl in purple.
(C) The
myristoyl binding pocket lies under the N-terminal amphiphilic
helix in the myr(−)-hARF·GDP structure (Protein Data
Bank ID code 1hur).
(D) The interstrand loop λ3 clashes
with the myristoyl chain in the GTP-bound conformation but not
in the GDP-bound conformation. The backbone of
myr(+)-yARF1·GDP is shown in ivory,
myr(−)-hARF1·GDP in blue, and hARF1Δ17·GTP in
green. λ3 of all molecules is shown in red, and myristoyl is
shown in purple.
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