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Figure 2.
Figure 2. Negative staining transmission electron
micrographs of human insulin fibres prepared under a variety of
conditions. Unless otherwise stated, all solutions contained 5
mg/ml human insulin. The conditions used were: (a) 0.004 M
H[2]SO[4] (pH 2.1), 70 °C; (b) 0.01 M HCl (pH 2.0), 70
°C; (c) H[3]PO[4] (pH 2.0), 90 °C; (d) 0.5 M citric acid
(pH 1.9), 90 °C; (e) 20 mg/ml bovine insulin in 8.3 M acetic
acid (pH 1.6), 37 °C; (f) 20% acetic acid (pH 2.0) and 0.004
M Na[2]SO[4], 90 °C.
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