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Figure 1.
FIGURE 1. Schematic representation of QAW strategy. A[1],
A[2], A[i], A[j], A[k], and A[n] represent the 1st, 2nd, i-th,
j-th, k-th, and n-th amino acids of the protein sequence with n
amino acids, respectively. The mutant data base at the i-th
position (i = 1, 2, i, j, k, and n) represents the effect of
amino acid replacement at the corresponding i-th position on a
specific function to be improved, such as activity,
conformational stability, thermal stability, etc. By looking at
each data base, three amino acids (at most) that represent the
top three scores in terms of the desired function are considered
for further mutations. The term quasi-additive is based on the
postulation of a rough (not strict) additivity of the adaptive
walking by combinatorial search for better mutant proteins.
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