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Figure 1.
Figure 1: Functional characterization of Na^+,K^+-ATPase used
for crystallization. a, The reaction cycle of the
Na^+,K^+-ATPase^2, ^3 showing in red the form crystallized. b,
Demonstration of ^86Rb^+ occlusion in the MgF[4]^2--bound form
of the pig renal Na^+,K^+-ATPase from outer medulla. The
dissociation of ^86Rb^+ from membranous enzyme pre-incubated
with ^86Rb^+ in the absence (lowest panel) or presence (middle
panel) of MgF[4]^2-, and from C[12]E[8]-solubilized enzyme
preincubated in the presence of MgF[4]^2- (uppermost panel), was
followed at 25 °C. Error bars, s.e.m.; n = 3.
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