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Figure 1.
Figure 1. Dpo4-catalyzed extension of a G  T
primer -terminal mispair. (a) The incorporation of dGTP
opposite C following an A-T primer -terminal base pair (left) is
500-fold more efficient when compared to the incorporation of
dGTP opposite C following a G T
primer -terminal mispair (right). The incorporation of dGTP (200
 M)
was examined for the indicated time periods for each substrate.
The enzyme concentrations for the A-T base-paired and the G T
mispaired primer termini were 0.2 nM and 1.2 nM, respectively.
(b) The rate of dGTP incorporation opposite a template C
following an A-T primer -terminal base pair (left) or following
a G T
primer -terminal mispair (right) was graphed as a function of
dGTP concentration. The solid line represents the best fit to
the Michaelis-Menten equation. The steady-state parameters, k
[cat] and K [m], are listed in Table 1.
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