Figure 1.
Fig. 1. Posttranslational modifications revealed by
structures of GFP variants before and after backbone
cyclization. Omit |F[o] - F[c]| electron density maps for the
chromophore residues contoured at 3 (black). (a) The
1.50-Å cyclized R96A structure. (b) The 2.00-Å
precyclization R96A intermediate A structure. (c) The
2.00-Å precyclization R96A intermediate B structure. (d
and e) Orthogonal views of the 1.80-Å Gly-Gly-Gly aerobic
oxidized postcyclization structure. (f) Proposed molecular
structure of the Gly-Gly-Gly cyclized ring. (g) The 1.80-Å
Gly-Gly-Gly anaerobic precyclization structure. All are
illustrated inRASTER 3D (44).
(black). (a) The
1.50-Å cyclized R96A structure. (b) The 2.00-Å
precyclization R96A intermediate A structure. (c) The
2.00-Å precyclization R96A intermediate B structure. (d
and e) Orthogonal views of the 1.80-Å Gly-Gly-Gly aerobic
oxidized postcyclization structure. (f) Proposed molecular
structure of the Gly-Gly-Gly cyclized ring. (g) The 1.80-Å
Gly-Gly-Gly anaerobic precyclization structure. All are
illustrated inRASTER 3D (44).