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Figure 1.
Figure 1. FkpA prevents MalE31 aggregation. Cells
expressing chromosomally encoded MalE-wt or MalE31, transformed
by pTrc99 or pTfkp, were grown in rich medium at 37°C for 3 h,
and then fractionated from spheroplasts. Periplasmic (soluble)
and membrane (insoluble) fractions, corresponding to 5 x 10^9
cells, were analyzed on SDS-polyacrylamide (12.5%) gel stained
by Coomassie blue. (Lane 1), Strain PD28 (  malE)
carrying pTrc99. (Lane 2) Strain MC4100 (malE) carrying pTrc99.
(Lane 3) Strain JMB5 (malE31) carrying pTrc99. (Lane 4) JMB5
cells carrying pTfkp.
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