Figure 1 - full size

Figure 1.
Fig. 1. Acrylamide-quenched fluorescence spectra of Bj2S (295 nm excitation) showing a progressive blue shift in λ[max] with increasing concentration of acrylamide (acrylamide concentration for the most quenched spectrum is 0.26 M). Center of gravity of emission, λ[cg] (integrated λ[em]-weighted normalized fluorescence intensity), as a function of acrylamide concentration for 295 (○), 298 ( triangle, open ) and 300 ( open ) nm excitation are shown in the inset.