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Figure 1.
Figure 1. Structural overview. a, Schematic of the RNA
sequence showing the conserved nucleotides (boxed), tetraloop
(green) and bulged-G motif (yellow). The sites of 2'
modification of the stem (asterisks) and of 4325 and 4326 (plus
signs) are shown. The terminal nucleotides are designed to pair
and create a blunt end (red); the remainder corresponds to rat
28S nucleotides 4315 -4333 (hereafter, Escherichia coli 23S
numbering is in parentheses). The two 29-mer variants
(nU4325dA^4326 and mG4325dA^4326) are missing the first and the
last nucleotide. When enzyme concentrations exceed that of
substrates other than ribosomes, cleavage by the toxin is
primarily on the 3' side of purines3. b, A ribbon drawing of the
restrictocin -mG4325 (misdocked) complex showing the tetraloop
(green) and bulged-G motif (yellow) of the substrate, as well as
the bound potassium ions (pink).
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