_EC 4.2.2.1 hyaluronate lyase. 43 PDB entries  
EC 4.-.-.- Lyases. [10,232 PDB entries]
EC 4.2.-.- Carbon-oxygen lyases. [5,835 PDB entries]
EC 4.2.2.- Acting on polysaccharides. [319 PDB entries]
EC 4.2.2.1 hyaluronate lyase. [43 PDB entries]
1c82

Reaction: [hyaluronan](n) = n 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D- glucosamine + H2O.
 

[hyaluronan](n)
 =
n 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D- glucosamine
 + H2O
Molecule diagrams generated from .mol files obtained from the KEGG ftp site.

Other name(s): hyaluronidase. mucinase. spreading factor.
Comments: The enzyme catalyzes the degradation of hyaluronan by a beta- elimination reaction. Also acts on chondroitin. The product is more systematically known as 3-(4-deoxy-alpha-L-threo- hex-4-enopyranosyluronic acid)-2-acetamido-2-deoxy-D-glucose. Formerly Ec 4.2.99.1.
Links:   [IntEnz]   [ExPASy]   [KEGG]  

There are 43 PDB entries in enzyme class E.C.4.2.2.1

  PDB code Protein
1c82
Mechanism of hyaluronan binding and degradation: structure of streptococcus pneumoniae hyaluronate lyase in complex with hyaluronic acid disaccharide at 1.7 a resolution
Source: Streptococcus pneumoniae. Organism_taxid: 1313. Expressed in: escherichia coli bl21. Expression_system_taxid: 511693. Other_details: escherichia coli
Chain: A (719 residues) CATH domains: 2.70.98.10 1.50.10.100 2.60.220.10
1egu
Crystal structure of streptococcus pneumoniae hyaluronate lyase at 1.56 a resolution
Source: Streptococcus pneumoniae. Organism_taxid: 1313. Expressed in: escherichia coli bl21. Expression_system_taxid: 511693.
Chain: A (721 residues) CATH domains: 2.70.98.10 1.50.10.100 2.60.220.10
1f1s
Crystal structure of streptococcus agalactiae hyaluronate lyase at 2.1 angstrom resolution.
Source: Streptococcus agalactiae. Organism_taxid: 1311. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (814 residues) CATH domains: 2.70.98.10 1.50.10.100 2.60.220.10
1f9g
Crystal structure of streptococcus pneumoniae hyaluronate lyase cocrystallized with ascorbic acid
Source: Streptococcus pneumoniae. Organism_taxid: 1313. Strain: bl21. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (721 residues) CATH domains: 2.70.98.10 1.50.10.100 2.60.220.10
1i8q
Crystal structure of streptococcus agalactiae hyaluronate lyase complexed with enzyme product, unsaturated disaccharide hyaluronan
Source: Streptococcus agalactiae. Organism_taxid: 1311. Gene: hyl. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (814 residues) CATH domains: 2.70.98.10 1.50.10.100 2.60.220.10
1loh
Streptococcus pneumoniae hyaluronate lyase in complex with hexasaccharide hyaluronan substrate
Source: Streptococcus pneumoniae. Organism_taxid: 1313. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (721 residues) CATH domains: 2.70.98.10 1.50.10.100 2.60.220.10
Bound ligand:   Het Group NAG is 57.69% similar to enzyme product 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D- glucosamine
1lxk
Streptococcus pneumoniae hyaluronate lyase in complex with tetrasaccharide hyaluronan substrate
Source: Streptococcus pneumoniae. Organism_taxid: 1313. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chain: A (719 residues) CATH domains: 2.70.98.10 1.50.10.100 2.60.220.10
Bound ligand:   Het Group NAG is 57.69% similar to enzyme product 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D- glucosamine
1lxm
Crystal structure of streptococcus agalactiae hyaluronate lyase complexed with hexasaccharide unit of hyaluronan
Source: Streptococcus agalactiae. Organism_taxid: 1311. Gene: hyl. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (794 residues) CATH domains: 2.70.98.10 1.50.10.100 2.60.220.10 2.60.40.1380
Bound ligand:   Het Group NAG is 57.69% similar to enzyme product 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D- glucosamine
1n7n
Streptococcus pneumoniae hyaluronate lyase w292a mutant
Source: Streptococcus pneumoniae. Organism_taxid: 1313. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (721 residues) CATH domains: 2.70.98.10 1.50.10.100 2.60.220.10
1n7o
Streptococcus pneumoniae hyaluronate lyase f343v mutant
Source: Streptococcus pneumoniae. Organism_taxid: 1313. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (721 residues) CATH domains: 2.70.98.10 1.50.10.100 2.60.220.10
1n7p
Streptococcus pneumoniae hyaluronate lyase w292a/f343v double mutant
Source: Streptococcus pneumoniae. Organism_taxid: 1313. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (721 residues) CATH domains: 2.70.98.10 1.50.10.100 2.60.220.10
1n7q
Streptococcus pneumoniae hyaluronate lyase w291a/w292a double mutant complex with hyaluronan hexasacchride
Source: Streptococcus pneumoniae. Organism_taxid: 1313. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (721 residues) CATH domains: 2.70.98.10 1.50.10.100 2.60.220.10
1n7r
Streptococcus pneumoniae hyaluronate lyase w291a/w292a/f343v mutant complex with hexasaccharide hyaluronan
Source: Streptococcus pneumoniae. Organism_taxid: 1313. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (721 residues) CATH domains: 2.70.98.10 1.50.10.100 2.60.220.10
1ojm
Specificity and mechanism of streptococcus pneumoniae hyaluronate lyase: complex with unsulphated chondroitin disaccharide
Source: Streptococcus pneumoniae. Organism_taxid: 1313. Expressed in: escherichia coli. Expression_system_taxid: 511693.
Chain: A (722 residues) CATH domains: 2.70.98.10 1.50.10.100 2.60.220.10
1ojn
Specificity and mechanism of streptococcus pneumoniae hyaluronate lyase: complex of the tyr408phe mutant with 6-sulphated chondroitin disaccharide
Source: Streptococcus pneumoniae. Organism_taxid: 1313. Expressed in: escherichia coli. Expression_system_taxid: 511693.
Chain: A (723 residues) CATH domains: 2.70.98.10 1.50.10.100 2.60.220.10
1ojo
Specificity and mechanism of streptococcus pneumoniae hyaluronate lyase: complex of the tyr408phe mutant with 4-sulphated chondroitin disaccharide
Source: Streptococcus pneumoniae. Organism_taxid: 1313. Expressed in: escherichia coli. Expression_system_taxid: 511693.
Chain: A (722 residues) CATH domains: 2.70.98.10 1.50.10.100 2.60.220.10
1ojp
Specificity and mechanism of streptococcus pneumoniae hyaluronate lyase: complex with 6-sulphated chondroitin disaccharide
Source: Streptococcus pneumoniae. Organism_taxid: 1313. Expressed in: escherichia coli. Expression_system_taxid: 511693.
Chain: A (723 residues) CATH domains: 2.70.98.10 1.50.10.100 2.60.220.10
1w3y
Crystal structure of s. Pneumoniae hyaluronate lyase in complex with palmitoyl-vitamin c
Source: Streptococcus pneumoniae. Organism_taxid: 1313. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (721 residues) CATH domains: 2.70.98.10 1.50.10.100 2.60.220.10
Bound ligand:   Het Group PVC is 43.24% similar to enzyme product 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D- glucosamine
2brp
Crystal structure of s. Pneumoniae hyaluronate lyase in complex with w249b
Source: Streptococcus pneumoniae. Organism_taxid: 1313. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (721 residues) CATH domains: 1.50.10.100 2.70.98.10 2.60.220.10
2brv
Crystal structure of streptococcus pneumoniae hyaluronate lyase from 70percent saturated malonate.
Source: Streptococcus pneumoniae. Organism_taxid: 1313. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: X (714 residues) CATH domains: 1.50.10.100 2.70.98.10 2.60.220.10
2brw
Crystal structure of streptococcus pneumoniae hyaluronate lyase from 30percent pegmme.
Source: Streptococcus pneumoniae. Organism_taxid: 1313. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B (722 residues) CATH domains: 1.50.10.100 2.70.98.10 2.60.220.10
2c3f
The structure of a group a streptococcal phage-encoded tail-fibre showing hyaluronan lyase activity.
Source: Streptococcus pyogenes. Organism_taxid: 1314. Strain: m1 gas sf370. Atcc: 700294. Expressed in: escherichia coli. Expression_system_taxid: 511693.
Chain: A (322 residues)
2dp5
Structure of streptococcus pyogenes bacteriophage-associated hyaluronate lyase hylp2
Source: Streptococcus pyogenes. Organism_taxid: 1314. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (332 residues)
2wb3
The partial structure of a group a streptococcal phage-encoded tail fibre hyaluronate lyase hylp3
Source: Streptococcus pyogenes. Organism_taxid: 1314. Strain: m1 gas sf370. Atcc: 700294. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chain: A (155 residues)
2wda
The x-ray structure of the streptomyces coelicolor a3 chondroitin ac lyase in complex with chondroitin sulphate
Source: Streptomyces violaceoruber. Organism_taxid: 1935. Strain: a3(2). Expressed in: escherichia coli. Expression_system_taxid: 469008. Other_details: german collection of microorganisms (dsm) dsm no. 40783
Chain: A (742 residues) CATH domains: 1.50.10.100 2.70.98.10 2.60.220.10
2x03
The x-ray structure of the streptomyces coelicolor a3 chondroitin ac lyase y253a mutant
Source: Streptomyces coelicolor. Organism_taxid: 100226. Strain: a3(2). Expressed in: escherichia coli. Expression_system_taxid: 469008. Other_details: german collection of microorganisms (dsm) dsm no. 40783
Chains: A, B (742 residues) CATH domains: 1.50.10.100 2.70.98.10 2.60.220.10
2yvv
Crystal structure of hyluranidase complexed with lactose at 2.6 a resolution reveals three specific sugar recognition sites
Source: Streptococcus pyogenes. Organism_taxid: 1314. Gene: hyl p2. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (332 residues)
2yw0
Crystal structure of hyluranidase trimer at 2.6 a resolution
Source: Streptococcus pyogenes serotype m1. Organism_taxid: 301447. Strain: serotype m1. Gene: hyl p2. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (332 residues)
2yx2
Crystal structure of cloned trimeric hyluranidase from streptococcus pyogenes at 2.8 a resolution
Source: Streptococcus pyogenes. Organism_taxid: 1314. Gene: hyl p2. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (332 residues)
3eka
Crystal structure of the complex of hyaluranidase trimer with ascorbic acid at 3.1 a resolution reveals the locations of three binding sites
Source: Streptococcus pyogenes. Organism_taxid: 1314. Gene: hylp1, hylp2, spy_0701. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (332 residues)
4d0q
Hyaluronan binding module of the streptococcal pneumoniae hyaluronate lyase
Source: Streptococcus pneumoniae tigr4. Organism_taxid: 170187. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chain: A (161 residues) CATH domain: 2.60.120.260
7fhu
Crystal structure of multi-functional polysaccharide lyase smlt1473 from stenotrophomonas maltophilia (strain k279a) in apo form at ph 8.5
Source: Stenotrophomonas maltophilia k279a. Organism_taxid: 522373. Strain: k279a. Gene: smlt1473. Expressed in: escherichia coli bl21. Expression_system_taxid: 511693.
Chains: A, B (307 residues)
7fhv
Crystal structure of multi-functional polysaccharide lyase smlt1473 (wt) from stenotrophomonas maltophilia (strain k279a) in apo form at ph 6.5
Source: Stenotrophomonas maltophilia k279a. Organism_taxid: 522373. Strain: k279a. Gene: smlt1473. Expressed in: escherichia coli bl21. Expression_system_taxid: 511693.
Chains: A, B (307 residues)
7fhw
Crystal structure of multi-functional polysaccharide lyase smlt1473 from stenotrophomonas maltophilia (strain k279a) in apo form at ph 5.5
Source: Stenotrophomonas maltophilia k279a. Organism_taxid: 522373. Strain: k279a. Gene: smlt1473. Expressed in: escherichia coli bl21. Expression_system_taxid: 511693.
Chains: A, B (309 residues)
7fhx
Crystal structure of multi-functional polysaccharide lyase smlt1473 (wt) from stenotrophomonas maltophilia (strain k279a) in apo form at ph 5.0
Source: Stenotrophomonas maltophilia k279a. Organism_taxid: 522373. Strain: k279a. Gene: smlt1473. Expressed in: escherichia coli bl21. Expression_system_taxid: 511693.
Chains: A, B (309 residues)
7fhy
Crystal structure of multi-functional polysaccharide lyase smlt1473 (wt) from stenotrophomonas maltophilia (strain k279a) in apo form at ph 7.0
Source: Stenotrophomonas maltophilia k279a. Organism_taxid: 522373. Strain: k279a. Gene: smlt1473. Expressed in: escherichia coli bl21. Expression_system_taxid: 511693.
Chains: A, B (308 residues)
7fhz
Crystal structure of multi-functional polysaccharide lyase smlt1473 (wt) from stenotrophomonas maltophilia (strain k279a) in apo form at ph 9.0
Source: Stenotrophomonas maltophilia k279a. Organism_taxid: 522373. Strain: k279a. Gene: smlt1473. Expressed in: escherichia coli bl21. Expression_system_taxid: 511693.
Chain: A (308 residues)
7fi0
Crystal structure of multi-functional polysaccharide lyase smlt1473 (wt) from stenotrophomonas maltophilia (strain k279a) in mana bound form at ph-5.0
Source: Stenotrophomonas maltophilia k279a. Organism_taxid: 522373. Strain: k279a. Gene: smlt1473. Expressed in: escherichia coli bl21. Expression_system_taxid: 511693.
Chain: A (308 residues)
7fi1
Crystal structure of multi-functional polysaccharide lyase smlt1473 (wt) from stenotrophomonas maltophilia (strain k279a) in mana bound form at ph-7.0
Source: Stenotrophomonas maltophilia k279a. Organism_taxid: 522373. Strain: k279a. Gene: smlt1473. Expressed in: escherichia coli bl21. Expression_system_taxid: 511693.
Chain: A (308 residues)
7fi2
Crystal structure of multi-functional polysaccharide lyase smlt1473- h168a from stenotrophomonas maltophilia (strain k279a) at ph-5.0
Source: Stenotrophomonas maltophilia k279a. Organism_taxid: 522373. Strain: k279a. Gene: smlt1473. Expressed in: escherichia coli bl21. Expression_system_taxid: 511693.
Chain: A (308 residues)
8fnx
Crystal structure of hyaluronate lyase b from cutibacterium acnes
Source: Cutibacterium acnes hl110pa3. Organism_taxid: 765079. Strain: hl110pa3. Gene: ppa0380. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chain: A (762 residues)
8fyg
Crystal structure of hyaluronate lyase a from cutibacterium acnes
Source: Cutibacterium acnes hl043pa1. Organism_taxid: 765072. Strain: hl043pa1. Gene: hmpref9570_01449. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chains: A, B (762 residues)
8g0o
Crystal structure of y281f mutant of hyaluronate lyase b from cutibacterium acnes
Source: Cutibacterium acnes hl110pa3. Organism_taxid: 765079. Strain: hl110pa3. Gene: ppa0380. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chains: A, B (762 residues)