_EC 2.1.1.260 rRNA small subunit pseudouridine methyltransferase Nep1.

7 PDB entries

EC 2.-.-.- Transferases. [47,929 PDB entries] EC 2.1.-.- Transferring one-carbon groups. [5,200 PDB entries] EC 2.1.1.- Methyltransferases. [4,609 PDB entries] EC 2.1.1.260 rRNA small subunit pseudouridine methyltransferase Nep1. [7 PDB entries]

2v3j

Reaction:		pseudouridine(1191) in yeast 18S rRNA + S-adenosyl-L-methionine = N(1)- methylpseudouridine(1191) in yeast 18S rRNA + S-adenosyl-L-homocysteine + H(+).
		pseudouridine(1191) in yeast 18S rRNA + S-adenosyl-L-methionine = N(1)- methylpseudouridine(1191) in yeast 18S rRNA + S-adenosyl-L-homocysteine + H(+) Molecule diagrams generated from .mol files obtained from the KEGG ftp site.
Comments:		This enzyme, which occurs in both prokaryotes and eukaryotes, recognizes specific pseudouridine residues (Psi) in small subunits of ribosomal Rna based on the local rna structure. It recognizes Psi(914) in 16S rRNA from the archaeon Methanocaldococcus jannaschii, Psi(1191) in yeast 18S rRNA, and Psi(1248) in human 18S rRNA.
Links:		[IntEnz]   [ExPASy]   [KEGG]

There are 7 PDB entries in enzyme class E.C.2.1.1.260

PDB code		Protein
2v3j		The yeast ribosome synthesis factor emg1 alpha beta knot fold methyltransferase Source: Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 4932. Expressed in: escherichia coli. Expression_system_taxid: 562. Chain: A (217 residues) CATH domain: 3.40.1280.10
2v3k		The yeast ribosome synthesis factor emg1 alpha beta knot fold methyltransferase Source: Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 4932. Expressed in: escherichia coli. Expression_system_taxid: 562. Chain: A (218 residues) CATH domain: 3.40.1280.10 Bound ligand:   Het Group SAM corresponds to enzyme reactant S-adenosyl-L-methionine
3oii		Crystal structure of saccharomyces cerevisiae nep1/emg1 bound to s- adenosylhomocysteine Source: Saccharomyces cerevisiae. Yeast. Organism_taxid: 4932. Gene: emg1, nep1, ylr186w, l9470.5. Expressed in: escherichia coli. Expression_system_taxid: 511693. Chains: A, B (216 residues) CATH domain: 3.40.1280.10 Bound ligand:   Het Group SAH corresponds to enzyme product S-adenosyl-L-homocysteine
3oij		Crystal structure of saccharomyces cerevisiae nep1/emg1 bound to s- adenosylhomocysteine and 2 molecules of cognate RNA Source: Saccharomyces cerevisiae. Organism_taxid: 4932. Gene: emg1, nep1, ylr186w, l9470.5. Expressed in: escherichia coli. Expression_system_taxid: 511693. Synthetic: yes. Other_details: cognate RNA fragment synthesized to mimic natural RNA substrate Chains: A, B (211 residues) CATH domain: 3.40.1280.10 Bound ligand:   Het Group SAH is 96.15% similar to enzyme product S-adenosyl-L-homocysteine
3oin		Crystal structure of saccharomyces cerevisiae nep1/emg1 bound to s- adenosylhomocysteine and 1 molecule of cognate RNA Source: Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 4932. Gene: emg1, nep1, ylr186w, l9470.5. Expressed in: escherichia coli bl21. Expression_system_taxid: 511693. Synthetic: yes. Other_details: cognate RNA synthesized to mimic natural RNA substrate Chains: A, B (217 residues) CATH domain: 3.40.1280.10 Bound ligand:   Het Group SAH is 96.15% similar to enzyme product S-adenosyl-L-homocysteine
5jpq		Cryo-em structure of the 90s pre-ribosome Source: Chaetomium thermophilum. Organism_taxid: 209285. Saccharomyces cerevisiae (strain atcc 204508 / s288c). Baker's yeast. Organism_taxid: 559292. Organism_taxid: 209285 Chains: e, f (211 residues)
5tzs		Architecture of the yeast small subunit processome Source: Saccharomyces cerevisiae s288c. Yeast. Organism_taxid: 559292. Saccharomyces cerevisiae s288c, saccharomyces cerevisiae s288c. Organism_taxid: 559292 Chains: j, k (211 residues)