EC 1.7.2.2 - nitrite reductase (cytochrome; ammonia-forming).

_EC 1.7.2.2 nitrite reductase (cytochrome; ammonia-forming).

54 PDB entries

EC 1.-.-.- Oxidoreductases. [21,428 PDB entries]

EC 1.7.-.- Acting on other nitrogenous compounds as donors. [796 PDB entries]

EC 1.7.2.- With a cytochrome as acceptor. [420 PDB entries]

EC 1.7.2.2 nitrite reductase (cytochrome; ammonia-forming). [54 PDB entries]

1fs7

Reaction: 6 Fe(III)-[cytochrome c] + NH4(+) + 2 H2O = 6 Fe(II)-[cytochrome c] + nitrite + 8 H(+).

6 × Fe(III)-[cytochrome c]
+ NH4(+)
+ 2 × H2O
= 6 × Fe(II)-[cytochrome c]
+
nitrite
+ 8 × H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site.

Other name(s): cytochrome c552. cytochrome c nitrite reductase. multiheme nitrite reductase.

Comments: The enzyme also reduces nitric oxide and hydroxylamine to ammonia, and sulfite to sulfide.

Links: [IntEnz] [ExPASy] [KEGG]

There are 54 PDB entries in enzyme class E.C.1.7.2.2

PDB code Protein

1fs7

CytochromE C nitrite reductase from wolinella succinogenes

Source: Wolinella succinogenes. Organism_taxid: 844

Chain: A (471 residues) CATH domains: 1.10.1130.10 1.20.140.10

Bound ligand: Het Group HEC is 95.45% similar to enzyme cofactor heme b

1fs8

CytochromE C nitrite reductase from wolinella succinogenes-sulfate complex

Source: Wolinella succinogenes. Organism_taxid: 844

Chain: A (471 residues) CATH domains: 1.10.1130.10 1.20.140.10

Bound ligand: Het Group HEC is 95.45% similar to enzyme cofactor Heme b

1fs9

CytochromE C nitrite reductase from wolinella succinogenes-azide complex

Source: Wolinella succinogenes. Organism_taxid: 844

Chain: A (471 residues) CATH domains: 1.10.1130.10 1.20.140.10

Bound ligand: Het Group HEC is 95.45% similar to enzyme cofactor Heme b

1gu6

Structure of the periplasmic cytochromE C nitrite reductase from escherichia coli

Source: Escherichia coli. Organism_taxid: 562

Chains: A, C, E, G (441 residues) CATH domains: 1.10.1130.10 1.20.140.10

Bound ligand: Het Group HEC is 95.45% similar to enzyme cofactor heme b

1oah

CytochromE C nitrite reductase from desulfovibrio desulfuricans atcc 27774: the relevance of the two calcium sites in the structure of the catalytic subunit (nrfa).

Source: Desulfovibrio desulfuricans. Organism_taxid: 876. Atcc: 27774

Chains: A, B (475 residues) CATH domains: 1.10.1130.10 1.20.140.10

Bound ligand: Het Group HEC is 95.45% similar to enzyme cofactor heme b

1qdb

CytochromE C nitrite reductase

Source: Sulfurospirillum deleyianum. Organism_taxid: 65553. Cellular_location: periplasm

Chains: A, B, C (473 residues) CATH domains: 1.10.1130.10 1.20.140.10

Bound ligand: Het Group HEC is 95.45% similar to enzyme cofactor heme b

2e80

CytochromE C nitrite reductase from wolinella succinogenes with bound substrate nitrite

Source: Wolinella succinogenes. Organism_taxid: 273121. Strain: dsm 1740

Chain: A (471 residues) CATH domains: 1.10.1130.10 1.20.140.10

Bound ligands: Het Group NO2 corresponds to enzyme product nitrite

Het Group HEM is 95.45% similar to enzyme cofactor heme b

2e81

CytochromE C nitrite reductase from wolinella succinogenes with bound intermediate hydroxylamine

Source: Wolinella succinogenes. Organism_taxid: 273121. Strain: dsm 1740

Chain: A (471 residues) CATH domains: 1.10.1130.10 1.20.140.10

Bound ligands: Het Group HOA is 66.67% similar to enzyme product nitrite

Het Group HEM is 95.45% similar to enzyme cofactor heme b

2j7a

Crystal structure of cytochromE C nitrite reductase nrfha complex from desulfovibrio vulgaris

Source: Desulfovibrio vulgaris. Organism_taxid: 882. Strain: hildenborough. Atcc: 29579. Atcc: 29579

Chains: A, B, D, E, G, H, J, K, M, N, P, Q (495 residues) CATH domains: 1.10.1130.10 1.20.140.10

Bound ligand: Het Group HEC is 95.45% similar to enzyme cofactor heme b

2ot4

Structure of a hexameric multihemE C nitrite reductase from the extremophile bacterium thiolkalivibrio nitratireducens

Source: Thioalkalivibrio nitratireducens. Organism_taxid: 186931. Strain: alen 2

Chains: A, B (520 residues) CATH domains: 1.10.287.3080 1.10.1130.10 1.20.140.10

Bound ligand: Het Group HEC is 95.45% similar to enzyme cofactor heme b

2rdz

High resolution crystal structure of the escherichia coli cytochromE C nitrite reductase.

Source: Escherichia coli. Organism_taxid: 562. Strain: lcb2048. Other_details: periplasm

Chains: A, B, C, D (441 residues) CATH domains: 1.10.1130.10 1.20.140.10

Bound ligand: Het Group HEC is 95.45% similar to enzyme cofactor heme b

2rf7

Crystal structure of the escherichia coli nrfa mutant q263e

Source: Escherichia coli k12. Organism_taxid: 83333. Strain: k-12. Gene: nrfa. Expressed in: escherichia coli. Expression_system_taxid: 562.

Chains: A, B, C, D (441 residues) CATH domains: 1.10.1130.10 1.20.140.10

Bound ligand: Het Group HEC is 95.45% similar to enzyme cofactor heme b

2vr0

Crystal structure of cytochromE C nitrite reductase nrfha complex bound to the hqno inhibitor

Source: Desulfovibrio vulgaris. Organism_taxid: 882. Strain: hildenborough. Strain: hildenborough

Chains: A, B, D, E (495 residues) CATH domains: 1.10.1130.10 1.20.140.10

Bound ligand: Het Group HEC is 95.45% similar to enzyme cofactor heme b

2zo5

Structure of the thioalkalivibrio nitratireducens cytochromE C nitrite reductase in a complex with azide

Source: Thioalkalivibrio nitratireducens. Organism_taxid: 186931. Strain: alen 2

Chains: A, B (519 residues) CATH domains: 1.10.287.3080 1.10.1130.10 1.20.140.10

Bound ligand: Het Group HEC is 95.45% similar to enzyme cofactor heme b

3bnf

W. Succinogenes nrfa sulfite complex

Source: Wolinella succinogenes. Organism_taxid: 844

Chain: A (471 residues) CATH domains: 1.10.1130.10 1.20.140.10

Bound ligand: Het Group HEM is 95.45% similar to enzyme cofactor heme b

3bng

W. Succinogenes nrfa y218f

Source: Wolinella succinogenes. Organism_taxid: 844. Gene: nrfa y218f. Expressed in: wolinella succinogenes. Expression_system_taxid: 844

Chain: A (471 residues) CATH domains: 1.10.1130.10 1.20.140.10

Bound ligand: Het Group HEM is 95.45% similar to enzyme cofactor heme b

3bnh

W. Succinogenes nrfa y218f nitrite complex

Source: Wolinella succinogenes. Organism_taxid: 844. Gene: nrfa y218f. Expressed in: wolinella succinogenes. Expression_system_taxid: 844

Chain: A (471 residues) CATH domains: 1.10.1130.10 1.20.140.10

Bound ligands: Het Group NO2 corresponds to enzyme product nitrite

Het Group HEM is 95.45% similar to enzyme cofactor heme b

3bnj

W. Succinogenes nrfa y218f sulfite complex

Source: Wolinella succinogenes. Organism_taxid: 844. Gene: nrfa y218f. Expressed in: wolinella succinogenes. Expression_system_taxid: 844

Chain: A (471 residues) CATH domains: 1.10.1130.10 1.20.140.10

Bound ligand: Het Group HEM is 95.45% similar to enzyme cofactor heme b

3d1i

Structure of the thioalkalivibrio nitratireducens cytochromE C nitrite reductase in a complex with nitrite

Source: Thioalkalivibrio nitratireducens. Organism_taxid: 186931. Strain: alen 2

Chains: A, B (519 residues) CATH domains: 1.10.287.3080 1.10.1130.10 1.20.140.10

Bound ligands: Het Group NO2 corresponds to enzyme product nitrite

Het Group HEC is 95.45% similar to enzyme cofactor heme b

3f29

Structure of the thioalkalivibrio nitratireducens cytochromE C nitrite reductase in complex with sulfite

Source: Thioalkalivibrio nitratireducens. Organism_taxid: 186931. Strain: alen 2

Chains: A, B (519 residues) CATH domains: 1.10.287.3080 1.10.1130.10 1.20.140.10

Bound ligand: Het Group HEC is 95.45% similar to enzyme cofactor heme b

3fo3

Structure of the thioalkalivibrio nitratireducens cytochromE C nitrite reductase reduced by sodium dithionite (sulfite complex)

Source: Thioalkalivibrio nitratireducens. Organism_taxid: 186931. Strain: alen 2

Chains: A, B (519 residues) CATH domains: 1.10.287.3080 1.10.1130.10 1.20.140.10

Bound ligand: Het Group HEC is 95.45% similar to enzyme cofactor heme b

3gm6

Structure of the thioalkalivibrio nitratireducens cytochromE C nitrite reductase in complex with phosphate

Source: Thioalkalivibrio nitratireducens. Organism_taxid: 186931. Strain: alen 2

Chains: A, B (521 residues) CATH domains: 1.10.287.3080 1.10.1130.10 1.20.140.10

Bound ligand: Het Group HEC is 95.45% similar to enzyme cofactor heme b

3l1t

E. Coli nrfa sulfite ocmplex

Source: Escherichia coli. Organism_taxid: 83333. Strain: k-12, jcb2048

Chains: A, B, C, D (441 residues) CATH domains: 1.10.1130.10 1.20.140.10

Bound ligand: Het Group HEC is 95.45% similar to enzyme cofactor heme b

3lg1

Structure of the thioalkalivibrio nitratireducens cytochromE C nitrite reductase reduced by sodium borohydride (in complex with sulfite)

Source: Thioalkalivibrio nitratireducens. Organism_taxid: 186931. Strain: alen 2

Chains: A, B (519 residues) CATH domains: 1.10.287.3080 1.10.1130.10 1.20.140.10

Bound ligand: Het Group HEC is 95.45% similar to enzyme cofactor heme b

3lgq

Structure of the thioalkalivibrio nitratireducens cytochromE C nitrite reductase in complex with sulfite (modified tyr-303)

Source: Thioalkalivibrio nitratireducens. Organism_taxid: 186931. Strain: alen 2

Chains: A, B (519 residues) CATH domains: 1.10.287.3080 1.10.1130.10 1.20.140.10

Bound ligand: Het Group HEC is 95.45% similar to enzyme cofactor heme b

3mmo

Structure of the thioalkalivibrio nitratireducens cytochromE C nitrite reductase in complex with cyanide

Source: Thioalkalivibrio nitratireducens. Organism_taxid: 186931. Strain: alen 2

Chains: A, B (518 residues) CATH domains: 1.10.287.3080 1.10.1130.10 1.20.140.10

Bound ligand: Het Group HEC is 95.45% similar to enzyme cofactor heme b

3owm

Structure of the thioalkalivibrio nitratireducens cytochromE C nitrite reductase in a complex with hydroxylamine

Source: Thioalkalivibrio nitratireducens. Organism_taxid: 186931. Strain: alen 2

Chains: A, B (519 residues) CATH domains: 1.10.287.3080 1.10.1130.10 1.20.140.10

Bound ligands: Het Group NO2 is 66.67% similar to enzyme product nitrite

Het Group HEC is 95.45% similar to enzyme cofactor heme b

3rkh

Structure of the thioalkalivibrio nitratireducens cytochromE C nitrite reductase in a complex with nitrite (full occupancy)

Source: Thioalkalivibrio nitratireducens. Organism_taxid: 186931. Strain: alen 2

Chains: A, B (520 residues) CATH domains: 1.10.287.3080 1.10.1130.10 1.20.140.10

Bound ligands: Het Group NO2 corresponds to enzyme product nitrite

Het Group HEC is 95.45% similar to enzyme cofactor heme b

3s7w

Structure of the tvnirb form of thioalkalivibrio nitratireducens cytochromE C nitrite reductase with an oxidized gln360 in a complex with hydroxylamine

Source: Thioalkalivibrio nitratireducens. Organism_taxid: 186931. Strain: alen 2

Chains: A, B (519 residues) CATH domains: 1.10.287.3080 1.10.1130.10 1.20.140.10

Bound ligands: Het Group NO2 is 66.67% similar to enzyme product nitrite

Het Group HEC is 95.45% similar to enzyme cofactor heme b

3sce

Structure of the thioalkalivibrio nitratireducens cytochromE C nitrite reductase with a covalent bond between the ce1 atom of tyr303 and the cg atom of gln360 (tvnirb)

Source: Thioalkalivibrio nitratireducens. Organism_taxid: 186931. Strain: alen 2

Chains: A, B (519 residues) CATH domains: 1.10.287.3080 1.10.1130.10 1.20.140.10

Bound ligand: Het Group HEC is 95.45% similar to enzyme cofactor heme b

3sxq

Structure of a hexameric multihemE C nitrite reductase from the extremophile bacterium thiolkalivibrio paradoxus

Source: Thioalkalivibrio paradoxus. Organism_taxid: 108010. Strain: arh1

Chains: A, B (519 residues) CATH domains: 1.10.287.3080 1.10.1130.10 1.20.140.10

Bound ligand: Het Group HEC is 95.45% similar to enzyme cofactor heme b

3tor

Crystal structure of escherichia coli nrfa with europium bound

Source: Escherichia coli. Organism_taxid: 562. Strain: lcb 2048. Other_details: periplasm

Chains: A, B, C, D (441 residues) CATH domains: 1.10.1130.10 1.20.140.10

Bound ligand: Het Group HEC is 95.45% similar to enzyme cofactor heme b

3ttb

Structure of the thioalkalivibrio paradoxus cytochromE C nitrite reductase in complex with sulfite

Source: Thioalkalivibrio paradoxus. Organism_taxid: 108010. Strain: arh1

Chains: A, B (520 residues) CATH domains: 1.10.287.3080 1.10.1130.10 1.20.140.10

Bound ligand: Het Group HEC is 95.45% similar to enzyme cofactor heme b

3ubr

Laue structure of shewanella oneidensis cytochromE-C nitrite reductase

Source: Shewanella oneidensis. Organism_taxid: 70863. Gene: nrfa, shewanella oneidensis, so_3980. Expressed in: shewanella. Expression_system_taxid: 22

Chains: A, B (439 residues) CATH domains: 1.10.1130.10 1.20.140.10

Bound ligand: Het Group HEC is 95.45% similar to enzyme cofactor heme b

3uu9

Structure of the free tvnirb form of thioalkalivibrio nitratireducens cytochromE C nitrite reductase

Source: Thioalkalivibrio nitratireducens. Organism_taxid: 186931. Strain: alen 2

Chains: A, B (519 residues) CATH domains: 1.10.287.3080 1.10.1130.10 1.20.140.10

Bound ligand: Het Group HEC is 95.45% similar to enzyme cofactor heme b

3zow

Crystal structure of wild type nitrosomonas europaea cytochrome c552

Source: Nitrosomonas europaea. Organism_taxid: 915

Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R (81 residues) CATH domain: 1.10.760.10

Bound ligand: Het Group HEC is 95.45% similar to enzyme cofactor heme b

3zox

Crystal structure of n64del mutant of nitrosomonas europaea cytochrome c552 (monoclinic space group)

Source: Nitrosomonas europaea. Organism_taxid: 915. Expressed in: escherichia coli. Expression_system_taxid: 562.

Chains: A, B, C, D (80 residues) CATH domain: 1.10.760.10

Bound ligand: Het Group HEC is 95.45% similar to enzyme cofactor heme b

3zoy

Crystal structure of n64del mutant of nitrosomonas europaea cytochrome c552 (hexagonal space group)

Source: Nitrosomonas europaea. Organism_taxid: 915. Expressed in: escherichia coli. Expression_system_taxid: 562.

Chains: A, B, C, D (80 residues) CATH domain: 1.10.760.10

Bound ligand: Het Group HEC is 95.45% similar to enzyme cofactor heme b

4l38

Nitrite complex of tvnir, low dose data set

Source: Thioalkalivibrio nitratireducens. Organism_taxid: 1255043. Strain: dsm 14787 / uniqem 213 / alen2

Chains: A, B (520 residues) CATH domains: 1.10.287.3080 1.10.1130.10 1.20.140.10

Bound ligands: Het Group NO2 corresponds to enzyme product nitrite

Het Group HEC is 95.45% similar to enzyme cofactor heme b

4l3x

Nitrite complex of tvnir, first middle dose data set

Source: Thioalkalivibrio nitratireducens. Organism_taxid: 1255043. Strain: dsm 14787 / uniqem 213 / alen2

Chains: A, B (520 residues) CATH domains: 1.10.287.3080 1.10.1130.10 1.20.140.10

Bound ligands: Het Group NO2 corresponds to enzyme product nitrite

Het Group HEC is 95.45% similar to enzyme cofactor heme b

4l3y

Nitrite complex of tvnir, high dose data set (no complex)

Source: Thioalkalivibrio nitratireducens. Organism_taxid: 1255043. Strain: dsm 14787 / uniqem 213 / alen2

Chains: A, B (520 residues) CATH domains: 1.10.287.3080 1.10.1130.10 1.20.140.10

Bound ligands: Het Group NO2 corresponds to enzyme product nitrite

Het Group HEC is 95.45% similar to enzyme cofactor heme b

4l3z

Nitrite complex of tvnir, second middle dose data set

Source: Thioalkalivibrio nitratireducens. Organism_taxid: 1255043. Strain: dsm 14787 / uniqem 213 / alen2

Chains: A, B (520 residues) CATH domains: 1.10.287.3080 1.10.1130.10 1.20.140.10

Bound ligands: Het Group NO2 corresponds to enzyme product nitrite

Het Group HEC is 95.45% similar to enzyme cofactor heme b

4q0t

Free form of tvnir, low dose data set

Source: Thioalkalivibrio nitratireducens. Organism_taxid: 186931

Chains: A, B (520 residues) CATH domains: 1.10.287.3080 1.10.1130.10 1.20.140.10

Bound ligand: Het Group HEC is 95.45% similar to enzyme cofactor heme b

4q17

Free form of tvnir, middle dose data set

Source: Thioalkalivibrio nitratireducens. Organism_taxid: 186931

Chains: A, B (520 residues) CATH domains: 1.10.287.3080 1.10.1130.10 1.20.140.10

Bound ligand: Het Group HEC is 95.45% similar to enzyme cofactor heme b

4q1o

Free form of tvnir, high dose data set

Source: Thioalkalivibrio nitratireducens. Organism_taxid: 186931

Chains: A, B (520 residues) CATH domains: 1.10.287.3080 1.10.1130.10 1.20.140.10

Bound ligand: Het Group HEC is 95.45% similar to enzyme cofactor heme b

4q4u

Tvnir in complex with sulfite, low dose data set

Source: Thioalkalivibrio nitratireducens. Organism_taxid: 186931

Chains: A, B (519 residues) CATH domains: 1.10.287.3080 1.10.1130.10 1.20.140.10

Bound ligand: Het Group HEC is 95.45% similar to enzyme cofactor heme b

4q5b

Tvnir in complex with sulfite, high dose data set

Source: Thioalkalivibrio nitratireducens. Organism_taxid: 186931

Chains: A, B (519 residues) CATH domains: 1.10.287.3080 1.10.1130.10 1.20.140.10

Bound ligand: Het Group HEC is 95.45% similar to enzyme cofactor heme b

4q5c

Tvnir in complex with sulfite, middle dose data set

Source: Thioalkalivibrio nitratireducens. Organism_taxid: 186931

Chains: A, B (519 residues) CATH domains: 1.10.287.3080 1.10.1130.10 1.20.140.10

Bound ligand: Het Group HEC is 95.45% similar to enzyme cofactor heme b

4wjy

Esherichia coli nitrite reductase nrfa h264n

Source: Escherichia coli k-12. Organism_taxid: 83333. Gene: nrfa, b4070, jw4031. Expressed in: escherichia coli. Expression_system_taxid: 562.

Chains: A, B (441 residues) CATH domains: 1.10.1130.10 1.20.140.10

Bound ligand: Het Group HEC is 95.45% similar to enzyme cofactor heme b

6p73

CytochromE-C-nitrite reductase

Source: Shewanella oneidensis. Organism_taxid: 70863. Gene: nrfa, so_3980. Expressed in: escherichia coli. Expression_system_taxid: 562

Chains: A, B (439 residues)

Bound ligand: Het Group HEC is 95.45% similar to enzyme cofactor heme b

6v0a

Crystal structure of cytochromE C nitrite reductase from the bacterium geobacter lovleyi with bound sulfate

Source: Geobacter lovleyi (strain atcc baa-1151 / dsm 17278 / sz). Organism_taxid: 398767. Strain: atcc baa-1151 / dsm 17278 / sz. Variant: dsm 17278. Cell_line: sz. Atcc: baa-1151. Gene: glov_1042. Expressed in: shewanella oneidensis mr-1.

Chains: A, B, C, D, E, F (443 residues)

Bound ligand: Het Group HEC is 95.45% similar to enzyme cofactor heme b

8bcx

Crystal structure of nrfa-1 from geobacter metallireducens

Source: Geobacter metallireducens gs-15. Organism_taxid: 269799. Strain: atcc 53774 / dsm 7210 / gs-15. Atcc: 53774. Gene: nrfa-1, gmet_0294. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Expression_system_variant: c43

Chains: A, B, C, D (437 residues)

Bound ligand: Het Group HEC is 95.45% similar to enzyme cofactor heme b

8rv0

Crystal structure of octaheme nitrite reductase from trichlorobacter ammonificans in complex with nitrite

Source: Trichlorobacter ammonificans. Organism_taxid: 2916410

Chain: Y (495 residues)

9cuf

Room temperature ssx structure of ccnir

Source: Shewanella oneidensis. Organism_taxid: 70863. Gene: nrfa, so_3980. Expressed in: escherichia coli. Expression_system_taxid: 562

Chains: A, B (439 residues)

Bound ligand: Het Group HEC is 95.45% similar to enzyme cofactor heme b