_EC 1.5.1.54 methylenetetrahydrofolate reductase (NADH). 20 PDB entries  
EC 1.-.-.- Oxidoreductases. [21,435 PDB entries]
EC 1.5.-.- Acting on the CH-NH group of donors. [1,240 PDB entries]
EC 1.5.1.- With NAD(+) or NADP(+) as acceptor. [1,014 PDB entries]
EC 1.5.1.54 methylenetetrahydrofolate reductase (NADH). [20 PDB entries]
1b5t

Reaction: (6S)-5-methyl-5,6,7,8-tetrahydrofolate + NAD(+) = (6R)-5,10-methylene- 5,6,7,8-tetrahydrofolate + Nadh + H(+).
 

(6S)-5-methyl-5,6,7,8-tetrahydrofolate
 +
NAD(+)
 = (6R)-5,10-methylene- 5,6,7,8-tetrahydrofolate
 +
NADH
 + H(+)
Molecule diagrams generated from .mol files obtained from the KEGG ftp site.

Other name(s): 5,10-CH2-H4folate reductase. 5,10-methylenetetrahydrofolate reductase. 5,10-methylenetetrahydrofolate reductase (FADH2). N(5),10-methylenetetrahydrofolate reductase.
Comments: A flavoprotein (FAD). The enzyme, found in plants and some bacteria, catalyzes the reversible conversion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate using Nadh as the electron donor. It play an important role in folate metabolism by regulating the distribution of one-carbon moieties between cellular methylation reactions and nucleic acid synthesis. These proteins either contain a C-terminal domain that does not mediate allosteric regulation (as in plants) or lack this domain entirely (as in Escherichia coli).
Links:   [IntEnz]   [ExPASy]   [KEGG]  

There are 20 PDB entries in enzyme class E.C.1.5.1.54

  PDB code Protein
1b5t
Escherichia coli methylenetetrahydrofolate reductase
Source: Escherichia coli. Organism_taxid: 562. Gene: metf. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B, C (275 residues) CATH domain: 3.20.20.220
Bound ligand:   Het Group FAD is 76.36% similar to enzyme reactant NAD(+)
1v93
5,10-methylenetetrahydrofolate reductase from thermus thermophilus hb8
Source: Thermus thermophilus. Organism_taxid: 300852. Strain: hb8. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (292 residues) CATH domain: 3.20.20.220
Bound ligand:   Het Group FAD corresponds to enzyme cofactor FAD
1zp3
E. Coli methylenetetrahydrofolate reductase (oxidized)
Source: Escherichia coli. Organism_taxid: 562. Gene: metf. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C (284 residues) CATH domain: 3.20.20.220
Bound ligand:   Het Group FAD corresponds to enzyme cofactor FAD
1zp4
Glu28gln mutant of e. Coli methylenetetrahydrofolate reductase (oxidized) complex with methyltetrahydrofolate
Source: Escherichia coli. Organism_taxid: 562. Gene: metf. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C (282 residues) CATH domain: 3.20.20.220
Bound ligand:   Het Group FAD corresponds to enzyme cofactor FAD
1zpt
Escherichia coli methylenetetrahydrofolate reductase (reduced) complexed with nadh, ph 7.25
Source: Escherichia coli. Organism_taxid: 562. Gene: metf. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C (271 residues) CATH domain: 3.20.20.220
Bound ligands:   Het Group NAI is 91.67% similar to enzyme reactant NADP(+)
  Het Group NAI corresponds to enzyme reactant NAD(+)
  Het Group FAD corresponds to enzyme cofactor FAD
1zrq
Escherichia coli methylenetetrahydrofolate reductase (reduced) complexed with nadh, ph 6.0
Source: Escherichia coli. Organism_taxid: 562. Gene: metf. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C (281 residues) CATH domain: 3.20.20.220
Bound ligands:   Het Group NAI is 91.67% similar to enzyme reactant NADP(+)
  Het Group NAI corresponds to enzyme reactant NAD(+)
  Het Group FAD corresponds to enzyme cofactor FAD
2fmn
Ala177val mutant of e. Coli methylenetetrahydrofolate reductase complex with ly309887
Source: Escherichia coli. Organism_taxid: 562. Gene: metf. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C (288 residues) CATH domain: 3.20.20.220
Bound ligand:   Het Group FAD corresponds to enzyme cofactor FAD
2fmo
Ala177val mutant of e. Coli methylenetetrahydrofolate reductase
Source: Escherichia coli. Organism_taxid: 562. Gene: metf. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C (261 residues) CATH domain: 3.20.20.220
Bound ligand:   Het Group FAD corresponds to enzyme cofactor FAD
3apt
Properties and crystal structure of methylenetetrahydrofolate reductase from thermus thermophilus hb8
Source: Thermus thermophilus. Organism_taxid: 300852. Strain: hb8. Gene: ttha0327. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (292 residues) CATH domain: 3.20.20.220
Bound ligand:   Het Group FAD corresponds to enzyme cofactor FAD
3apy
Properties and crystal structure of methylenetetrahydrofolate reductase from thermus thermophilus hb8
Source: Thermus thermophilus. Organism_taxid: 300852. Strain: hb8. Gene: ttha0327. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D, E, F, G, H (291 residues) CATH domain: 3.20.20.220
Bound ligand:   Het Group FAD corresponds to enzyme cofactor FAD
3fst
Crystal structure of escherichia coli methylenetetrahydrofolate reductase mutant phe223leu at ph 7.4
Source: Escherichia coli k-12. Organism_taxid: 83333. Gene: metf, b3941, jw3913. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, C, E (286 residues) CATH domain: 3.20.20.220
Bound ligand:   Het Group FAD corresponds to enzyme cofactor FAD
3fsu
Crystal structure of escherichia coli methylenetetrahydrofolate reductase double mutant phe223leuglu28gln complexed with methyltetrahydrofolate
Source: Escherichia coli k-12. Organism_taxid: 83333. Gene: metf, b3941, jw3913. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, C, E (285 residues) CATH domain: 3.20.20.220
Bound ligand:   Het Group FAD corresponds to enzyme cofactor FAD
5ume
Crystal structure of 5,10-methylenetetrahydrofolate reductase metf from haemophilus influenzae
Source: Haemophilus influenzae (strain atcc 51907 / dsm 11121 / kw20 / rd). Organism_taxid: 71421. Strain: atcc 51907 / dsm 11121 / kw20 / rd. Gene: metf, hi_1444. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chains: A, B, C, D, E, F (285 residues)
Bound ligand:   Het Group FAD corresponds to enzyme cofactor FAD
6pey
Mthfr with mutation asp120ala
Source: Escherichia coli. Organism_taxid: 562. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B, C (287 residues)
Bound ligand:   Het Group FAD corresponds to enzyme cofactor FAD
7rml
Neisseria meningitidis methylenetetrahydrofolate reductase in complex with fad
Source: Neisseria meningitidis serogroup b (strain mc58). Organism_taxid: 122586. Strain: mc58. Gene: metf, nmb0943. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008
Chains: A, B, C (281 residues)
Bound ligand:   Het Group FAD corresponds to enzyme cofactor FAD
7th4
T. Thermophilus methylenetetrahydrofolate reductase complex with 5- formyltetrahydrofolate
Source: Thermus thermophilus hb8. Organism_taxid: 300852. Strain: atcc 27634 / dsm 579 / hb8. Gene: ttha0327. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B (291 residues)
Bound ligand:   Het Group FAD corresponds to enzyme cofactor FAD
7th5
Thermus thermophilus methylenetetrahydrofolate reductase
Source: Thermus thermophilus hb8. Organism_taxid: 300852. Strain: atcc 27634 / dsm 579 / hb8. Gene: ttha0327. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B (291 residues)
Bound ligand:   Het Group FAD corresponds to enzyme cofactor FAD
7xg9
Crystal structural of methylenetetrahydrofolate reductase from sphingobium sp. Syk-6
Source: Sphingobium sp. Syk-6. Organism_taxid: 627192. Strain: nbrc 103272 / syk-6. Gene: metf, slg_12750. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chains: A, B (286 residues)
Bound ligand:   Het Group FAD is 76.36% similar to enzyme reactant NAD(+)
7xlf
Crystal structure of ch3-thf complex of methylenetetrahydrofolate reductase from sphingobium sp. Syk-6
Source: Sphingobium sp. Syk-6. Organism_taxid: 627192. Strain: nbrc 103272 / syk-6. Gene: metf, slg_12750. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008
Chains: A, B (282 residues)
Bound ligand:   Het Group FAD corresponds to enzyme cofactor FAD
8eac
Thermus thermophilus methylenetetrahydrofolate reductase
Source: Thermus thermophilus hb8. Organism_taxid: 300852. Strain: atcc 27634 / dsm 579 / hb8. Gene: ttha0327. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B (287 residues)
Bound ligand:   Het Group FAD corresponds to enzyme cofactor FAD