EC 1.14.13.2 - 4-hydroxybenzoate 3-monooxygenase.

_EC 1.14.13.2 4-hydroxybenzoate 3-monooxygenase.

37 PDB entries

EC 1.-.-.- Oxidoreductases. [21,484 PDB entries]

EC 1.14.-.- Acting on paired donors, with incorporation or reduction of molecu [4,719 PDB entries]

EC 1.14.13.- With Nadh or nadph as one donor, and incorporation of one atom of [1,071 PDB entries]

EC 1.14.13.2 4-hydroxybenzoate 3-monooxygenase. [37 PDB entries]

1bf3

Pathway: Benzoate Metabolism

Reaction: 4-hydroxybenzoate + Nadph + O2 + H(+) = 3,4-dihydroxybenzoate + NADP(+) + H2O.

4-hydroxybenzoate
+
NADPH
+
O2
+ H(+)
=
3,4-dihydroxybenzoate
+
NADP(+)
+ H2O

Molecule diagrams generated from .mol files obtained from the KEGG ftp site.

Other name(s): para-hydroxybenzoate hydroxylase. p-hydroxybenzoate hydroxylase. p-hydroxybenzoic acid hydroxylase.

Comments: Most enzymes from Pseudomonas are highly specific for NAD(P)H (cf. Ec 1.14.13.33).

Links: [IntEnz] [ExPASy] [KEGG]

There are 37 PDB entries in enzyme class E.C.1.14.13.2

PDB code Protein

1bf3

P-hydroxybenzoate hydroxylase (phbh) mutant with cys 116 replaced by ser (c116s) and arg 42 replaced by lys (r42k), in complex with fad and 4-hydroxybenzoic acid

Source: Pseudomonas fluorescens. Organism_taxid: 294. Gene: poba. Expressed in: escherichia coli. Expression_system_taxid: 562.

Chain: A (391 residues) CATH domains: 3.50.50.60 3.30.9.10

Bound ligands: Het Group PHB corresponds to enzyme reactant 4-hydroxybenzoate

Het Group FAD corresponds to enzyme cofactor FAD

1bgj

P-hydroxybenzoate hydroxylase (phbh) mutant with cys 116 replaced by ser (c116s) and his 162 replaced by arg (h162r), in complex with fad and 4-hydroxybenzoic acid

Source: Pseudomonas fluorescens. Organism_taxid: 294. Gene: poba. Expressed in: escherichia coli. Expression_system_taxid: 562.

Chain: A (391 residues) CATH domains: 3.50.50.60 3.30.9.10

Bound ligands: Het Group PHB corresponds to enzyme reactant 4-hydroxybenzoate

Het Group FAD corresponds to enzyme cofactor FAD

1bgn

P-hydroxybenzoate hydroxylase (phbh) mutant with cys 116 replaced by ser (c116s) and arg 269 replaced by thr (r269t), in complex with fad and 4-hydroxybenzoic acid

Source: Pseudomonas fluorescens. Organism_taxid: 294. Gene: poba. Expressed in: escherichia coli. Expression_system_taxid: 562.

Chain: A (391 residues) CATH domains: 3.50.50.60 3.30.9.10

Bound ligands: Het Group PHB corresponds to enzyme reactant 4-hydroxybenzoate

Het Group FAD corresponds to enzyme cofactor FAD

1bkw

P-hydroxybenzoate hydroxylase (phbh) mutant with cys116 replaced by ser (c116s) and arg44 replaced by lys (r44k), in complex with fad and 4-hydroxybenzoic acid

Source: Pseudomonas fluorescens. Organism_taxid: 294. Expressed in: escherichia coli. Expression_system_taxid: 562.

Chain: A (391 residues) CATH domains: 3.50.50.60 3.30.9.10

Bound ligands: Het Group PHB corresponds to enzyme reactant 4-hydroxybenzoate

Het Group FAD corresponds to enzyme cofactor FAD

1cc4

Phe161 and arg166 variants of p-hydroxybenzoate hydroxylase. Implications for NADPH recognition and structural stability.

Source: Pseudomonas fluorescens. Organism_taxid: 294. Strain: tg2. Gene: poba. Expressed in: escherichia coli. Expression_system_taxid: 562.

Chain: A (391 residues) CATH domains: 3.50.50.60 3.30.9.10

Bound ligands: Het Group PHB corresponds to enzyme reactant 4-hydroxybenzoate

Het Group FAD corresponds to enzyme cofactor FAD

1cc6

Phe161 and arg166 variants of p-hydroxybenzoate hydroxylase. Implications for NADPH recognition and structural stability.

Source: Pseudomonas fluorescens. Organism_taxid: 294. Strain: tg2. Gene: poba. Expressed in: escherichia coli. Expression_system_taxid: 562.

Chain: A (391 residues) CATH domains: 3.50.50.60 3.30.9.10

Bound ligands: Het Group PHB corresponds to enzyme reactant 4-hydroxybenzoate

Het Group FAD corresponds to enzyme cofactor FAD

1cj2

Mutant gln34arg of para-hydroxybenzoate hydroxylase

Source: Pseudomonas fluorescens. Organism_taxid: 294. Gene: poba. Expressed in: escherichia coli. Expression_system_taxid: 562.

Chain: A (391 residues) CATH domains: 3.50.50.60 3.30.9.10

Bound ligands: Het Group PHB corresponds to enzyme reactant 4-hydroxybenzoate

Het Group FAD corresponds to enzyme cofactor FAD

1cj3

Mutant tyr38glu of para-hydroxybenzoate hydroxylase

Source: Pseudomonas fluorescens. Organism_taxid: 294. Gene: poba. Expressed in: escherichia coli. Expression_system_taxid: 562.

Chain: A (392 residues) CATH domains: 3.50.50.60 3.30.9.10

Bound ligands: Het Group PHB corresponds to enzyme reactant 4-hydroxybenzoate

Het Group FAD corresponds to enzyme cofactor FAD

1cj4

Mutant q34t of para-hydroxybenzoate hydroxylase

Source: Pseudomonas fluorescens. Organism_taxid: 294. Gene: poba. Expressed in: escherichia coli. Expression_system_taxid: 562.

Chain: A (392 residues) CATH domains: 3.50.50.60 3.30.9.10

Bound ligands: Het Group PHB corresponds to enzyme reactant 4-hydroxybenzoate

Het Group FAD corresponds to enzyme cofactor FAD

1d7l

Structure-function correlations of the reaction of reduced nicotinamide analogs with p-hydroxybenzoate hydroxylase substituted with a series of 8-substituted flavins

Source: Pseudomonas aeruginosa. Organism_taxid: 287. Expressed in: escherichia coli. Expression_system_taxid: 562.

Chain: A (394 residues) CATH domains: 3.50.50.60 3.30.9.10

Bound ligands: Het Group PHB corresponds to enzyme reactant 4-hydroxybenzoate

Het Group RFL is 92.86% similar to enzyme cofactor FAD

1dob

The mobil flavin of 4-oh benzoate hydroxylase: motion of a prosthetic group regulates catalysis

Source: Pseudomonas aeruginosa. Organism_taxid: 287

Chain: A (394 residues) CATH domains: 3.50.50.60 3.30.9.10

Bound ligands: Het Group PHB corresponds to enzyme reactant 4-hydroxybenzoate

Het Group FAD corresponds to enzyme cofactor FAD

1doc

The mobil flavin of 4-oh benzoate hydroxylase: motion of a prosthetic group regulates catalysis

Source: Pseudomonas aeruginosa. Organism_taxid: 287

Chain: A (394 residues) CATH domains: 3.50.50.60 3.30.9.10

Bound ligands: Het Group PHB corresponds to enzyme reactant 4-hydroxybenzoate

Het Group FAD corresponds to enzyme cofactor FAD

1dod

The mobil flavin of 4-oh benzoate hydroxylase: motion of a prosthetic group regulates catalysis

Source: Pseudomonas aeruginosa. Organism_taxid: 287

Chain: A (394 residues) CATH domains: 3.50.50.60 3.30.9.10

Bound ligands: Het Group DOB is 90.91% similar to enzyme reactant 4-hydroxybenzoate

Het Group FAD corresponds to enzyme cofactor FAD

1doe

The mobil flavin of 4-oh benzoate hydroxylase: motion of a prosthetic group regulates catalysis

Source: Pseudomonas aeruginosa. Organism_taxid: 287

Chain: A (394 residues) CATH domains: 3.50.50.60 3.30.9.10

Bound ligands: Het Group DOB is 90.91% similar to enzyme reactant 4-hydroxybenzoate

Het Group FAD corresponds to enzyme cofactor FAD

1ius

P-hydroxybenzoate hydroxylase complexed with 4-aminobenzoate at ph 5.0

Source: Pseudomonas aeruginosa. Organism_taxid: 287

Chain: A (394 residues) CATH domains: 3.50.50.60 3.30.9.10

Bound ligands: Het Group PAB is 81.82% similar to enzyme reactant 4-hydroxybenzoate

Het Group FAD corresponds to enzyme cofactor FAD

1iut

P-hydroxybenzoate hydroxylase complexed with 4-aminobenzoate at ph 7.4

Source: Pseudomonas aeruginosa. Organism_taxid: 287

Chain: A (394 residues) CATH domains: 3.50.50.60 3.30.9.10

Bound ligands: Het Group PAB is 81.82% similar to enzyme reactant 4-hydroxybenzoate

Het Group FAD corresponds to enzyme cofactor FAD

1iuu

P-hydroxybenzoate hydroxylase complexed with 4-aminobenzoate at ph 9.4

Source: Pseudomonas aeruginosa. Organism_taxid: 287

Chain: A (394 residues) CATH domains: 3.50.50.60 3.30.9.10

Bound ligands: Het Group PAB is 81.82% similar to enzyme reactant 4-hydroxybenzoate

Het Group FAD corresponds to enzyme cofactor FAD

1iuv

P-hydroxybenzoate hydroxylase complexed with 4-4-hydroxybenzoate at ph 5.0

Source: Pseudomonas aeruginosa. Organism_taxid: 208964. Strain: pao1. Gene: poba. Expressed in: escherichia coli. Expression_system_taxid: 562.

Chain: A (394 residues) CATH domains: 3.50.50.60 3.30.9.10

Bound ligands: Het Group PHB corresponds to enzyme reactant 4-hydroxybenzoate

Het Group FAD corresponds to enzyme cofactor FAD

1iuw

P-hydroxybenzoate hydroxylase complexed with 4-4-hydroxybenzoate at ph 7.4

Source: Pseudomonas aeruginosa. Organism_taxid: 208964. Strain: pao1. Gene: poba. Expressed in: escherichia coli. Expression_system_taxid: 562.

Chain: A (394 residues) CATH domains: 3.50.50.60 3.30.9.10

Bound ligands: Het Group PHB corresponds to enzyme reactant 4-hydroxybenzoate

Het Group FAD corresponds to enzyme cofactor FAD

1iux

P-hydroxybenzoate hydroxylase complexed with 4-4-hydroxybenzoate at ph 9.4

Source: Pseudomonas aeruginosa. Organism_taxid: 208964. Strain: pao1. Gene: poba. Expressed in: escherichia coli. Expression_system_taxid: 562.

Chain: A (394 residues) CATH domains: 3.50.50.60 3.30.9.10

Bound ligands: Het Group PHB corresponds to enzyme reactant 4-hydroxybenzoate

Het Group FAD corresponds to enzyme cofactor FAD

1k0i

Pseudomonas aeruginosa phbh r220q in complex with 100mm phb

Source: Pseudomonas aeruginosa. Organism_taxid: 287. Expressed in: escherichia coli. Expression_system_taxid: 562.

Chain: A (394 residues) CATH domains: 3.50.50.60 3.30.9.10

Bound ligands: Het Group PHB corresponds to enzyme reactant 4-hydroxybenzoate

Het Group FAD corresponds to enzyme cofactor FAD

1k0j

Pseudomonas aeruginosa phbh r220q in complex with NADPH and free of p- ohb

Source: Pseudomonas aeruginosa. Organism_taxid: 287. Expressed in: escherichia coli. Expression_system_taxid: 562.

Chain: A (394 residues) CATH domains: 3.50.50.60 3.30.9.10

Bound ligands: Het Group NDP corresponds to enzyme reactant NADPH

Het Group FAD corresponds to enzyme cofactor FAD

1k0l

Pseudomonas aeruginosa phbh r220q free of p-ohb

Source: Pseudomonas aeruginosa. Organism_taxid: 287. Expressed in: escherichia coli. Expression_system_taxid: 562.

Chain: A (394 residues) CATH domains: 3.50.50.60 3.30.9.10

Bound ligand: Het Group FAD corresponds to enzyme cofactor FAD

1pbb

Crystal structures of wild-type p-hydroxybenzoate hydroxylase complexed with 4-aminobenzoate, 2,4-dihydroxybenzoate and 2-hydroxy- 4-aminobenzoate and of the try222ala mutant, complexed with 2- hydroxy-4-aminobenzoate. Evidence for a proton channel and a new binding mode of the flavin ring

Source: Pseudomonas fluorescens. Organism_taxid: 294

Chain: A (391 residues) CATH domains: 3.50.50.60 3.30.9.10

Bound ligands: Het Group DOB is 90.91% similar to enzyme reactant 4-hydroxybenzoate

Het Group FAD corresponds to enzyme cofactor FAD

1pbc

Crystal structures of wild-type p-hydroxybenzoate hydroxylase complexed with 4-aminobenzoate, 2,4-dihydroxybenzoate and 2-hydroxy- 4-aminobenzoate and of the try222ala mutant, complexed with 2- hydroxy-4-aminobenzoate. Evidence for a proton channel and a new binding mode of the flavin ring

Source: Pseudomonas fluorescens. Organism_taxid: 294

Chain: A (391 residues) CATH domains: 3.50.50.60 3.30.9.10

Bound ligands: Het Group BHA is 75.00% similar to enzyme reactant 4-hydroxybenzoate

Het Group FAD corresponds to enzyme cofactor FAD

1pbd

Crystal structures of wild-type p-hydroxybenzoate hydroxylase complexed with 4-aminobenzoate, 2,4-dihydroxybenzoate and 2-hydroxy- 4-aminobenzoate and of the try222ala mutant, complexed with 2- hydroxy-4-aminobenzoate. Evidence for a proton channel and a new binding mode of the flavin ring

Source: Pseudomonas fluorescens. Organism_taxid: 294

Chain: A (391 residues) CATH domains: 3.50.50.60 3.30.9.10

Bound ligands: Het Group PAB is 81.82% similar to enzyme reactant 4-hydroxybenzoate

Het Group FAD corresponds to enzyme cofactor FAD

1pbe

Crystal structure of the p-hydroxybenzoate hydroxylase-substrate complex refined at 1.9 angstroms resolution. Analysis of the enzyme- substrate and enzyme-product complexes

Source: Pseudomonas fluorescens. Organism_taxid: 294

Chain: A (391 residues) CATH domains: 3.50.50.60 3.30.9.10

Bound ligands: Het Group PHB corresponds to enzyme reactant 4-hydroxybenzoate

Het Group FAD corresponds to enzyme cofactor FAD

1pbf

Crystal structures of wild-type p-hydroxybenzoate hydroxylase complexed with 4-aminobenzoate, 2,4-dihydroxybenzoate and 2-hydroxy- 4-aminobenzoate and of the try222ala mutant, complexed with 2- hydroxy-4-aminobenzoate. Evidence for a proton channel and a new binding mode of the flavin ring

Source: Pseudomonas fluorescens. Organism_taxid: 294

Chain: A (391 residues) CATH domains: 3.50.50.60 3.30.9.10

Bound ligands: Het Group BHA is 75.00% similar to enzyme reactant 4-hydroxybenzoate

Het Group FAD corresponds to enzyme cofactor FAD

1pdh

Crystal structure of p-hydroxybenzoate hydroxylase reconstituted with the modified fad present in alcohol oxidase from methylotrophic yeasts: evidence for an arabinoflavin

Source: Pseudomonas fluorescens. Organism_taxid: 294

Chain: A (391 residues) CATH domains: 3.50.50.60 3.30.9.10

Bound ligands: Het Group PHB corresponds to enzyme reactant 4-hydroxybenzoate

Het Group FAS corresponds to enzyme cofactor FAD

1phh

Crystal structure of p-hydroxybenzoate hydroxylase complexed with its reaction product 3,4-dihydroxybenzoate

Source: Pseudomonas fluorescens. Organism_taxid: 294

Chain: A (394 residues) CATH domains: 3.50.50.60 3.30.9.10

Bound ligands: Het Group DHB corresponds to enzyme product 3,4-dihydroxybenzoate

Het Group FAD corresponds to enzyme cofactor FAD

1pxa

Crystal structures of mutant pseudomonas aeruginosa p-hydroxybenzoate hydroxylase: the tyr201phe, tyr385phe, and asn300asp variants

Source: Pseudomonas aeruginosa. Organism_taxid: 287

Chain: A (394 residues) CATH domains: 3.50.50.60 3.30.9.10

Bound ligands: Het Group PHB corresponds to enzyme reactant 4-hydroxybenzoate

Het Group FAD corresponds to enzyme cofactor FAD

1pxb

Crystal structures of mutant pseudomonas aeruginosa p-hydroxybenzoate hydroxylase: the tyr201phe, tyr385phe, and asn300asp variants

Source: Pseudomonas aeruginosa. Organism_taxid: 287

Chain: A (394 residues) CATH domains: 3.50.50.60 3.30.9.10

Bound ligands: Het Group PHB corresponds to enzyme reactant 4-hydroxybenzoate

Het Group FAD corresponds to enzyme cofactor FAD

1pxc

Crystal structures of mutant pseudomonas aeruginosa p-hydroxybenzoate hydroxylase: the tyr201phe, tyr385phe, and asn300asp variants

Source: Pseudomonas aeruginosa. Organism_taxid: 287

Chain: A (394 residues) CATH domains: 3.50.50.60 3.30.9.10

Bound ligands: Het Group PHB corresponds to enzyme reactant 4-hydroxybenzoate

Het Group FAD corresponds to enzyme cofactor FAD

1ykj

A45g p-hydroxybenzoate hydroxylase with p-hydroxybenzoate bound

Source: Pseudomonas aeruginosa. Organism_taxid: 287. Gene: poba. Expressed in: escherichia coli. Expression_system_taxid: 562.

Chains: A, B (394 residues) CATH domains: 3.50.50.60 3.30.9.10

Bound ligands: Het Group PHB corresponds to enzyme reactant 4-hydroxybenzoate

Het Group FAD corresponds to enzyme cofactor FAD

2phh

The coenzyme analogue adenosine 5-diphosphoribose displaces fad in the active site of p-hydroxybenzoate hydroxylase. An x-ray crystallographic investigation

Source: Pseudomonas fluorescens. Organism_taxid: 294

Chain: A (391 residues) CATH domains: 3.50.50.60 3.30.9.10

Bound ligands: Het Group PHB corresponds to enzyme reactant 4-hydroxybenzoate

Het Group APR is 71.43% similar to enzyme reactant NADPH

6dll

2.2 angstrom resolution crystal structure of p-hydroxybenzoate hydroxylase from pseudomonas putida in complex with fad.

Source: Pseudomonas putida (strain atcc 47054 / dsm 6125 / ncimb 11950 / kt2440). Organism_taxid: 160488. Strain: atcc 47054 / dsm 6125 / ncimb 11950 / kt2440. Gene: poba, pp_3537. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Chains: A, B, C, D (397 residues)

Bound ligand: Het Group FAD corresponds to enzyme cofactor FAD

6ju1

P-hydroxybenzoate hydroxylase y385f mutant complexed with 3,4- dihydroxybenzoate

Source: Pseudomonas aeruginosa. Organism_taxid: 287. Gene: poba, dt376_03235. Expressed in: escherichia coli. Expression_system_taxid: 562.

Chain: A (393 residues)

Bound ligands: Het Group DHB corresponds to enzyme product 3,4-dihydroxybenzoate

Het Group FAD corresponds to enzyme cofactor FAD