_EC 1.12.98.2 5,10-methenyltetrahydromethanopterin hydrogenase. 18 PDB entries  
EC 1.-.-.- Oxidoreductases. [21,484 PDB entries]
EC 1.12.-.- Acting on hydrogen as donors. [251 PDB entries]
EC 1.12.98.- With other, known, acceptors. [30 PDB entries]
EC 1.12.98.2 5,10-methenyltetrahydromethanopterin hydrogenase. [18 PDB entries]
2b0j

Reaction: 5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2 = 5,10- methylenetetrahydromethanopterin + H(+).
 


5,10-methenyl-5,6,7,8-tetrahydromethanopterin
 + H2
 =
5,10- methylenetetrahydromethanopterin
 + H(+)
Molecule diagrams generated from .mol files obtained from the KEGG ftp site.

Other name(s): H2-dependent methylene-H4MPT dehydrogenase. H2-forming N(5),N(10)-methylenetetrahydromethanopterin dehydrogenase. hydrogen:N(5),N(10)-methenyltetrahydromethanopterin oxidoreductase. N(5),N(10)-methenyltetrahydromethanopterin hydrogenase. nonmetal hydrogenase.
Comments: Does not catalyze the reduction of artificial dyes. Does not by itself catalyze a H2/H(+) exchange reaction. Does not contain nickel or iron-sulfur clusters. Formerly Ec 1.12.99.4.
Links:   [IntEnz]   [ExPASy]   [KEGG]  

There are 18 PDB entries in enzyme class E.C.1.12.98.2

  PDB code Protein
2b0j
The crystal structure of the apoenzyme of the iron-sulfur-cluster-free hydrogenase (hmd)
Source: Methanocaldococcus jannaschii. Organism_taxid: 2190. Strain: dsmz2661. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (344 residues) CATH domains: 3.40.50.720 1.20.120.1300
3daf
The crystal structure of [fe]-hydrogenase holoenzyme (hmd) from methanocaldococcus jannaschii cocrystallized with cyanide
Source: Methanocaldococcus jannaschii. Methanococcus jannaschii. Organism_taxid: 2190. Strain: dsmz2661. Gene: hmd, mj0784. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (344 residues) CATH domains: 3.40.50.720 1.20.120.1300
3dag
The crystal structure of [fe]-hydrogenase holoenzyme (hmd) from methanocaldococcus jannaschii
Source: Methanocaldococcus jannaschii. Methanococcus jannaschii. Organism_taxid: 2190. Strain: dsmz2661. Gene: hmd, mj0784. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (344 residues) CATH domains: 3.40.50.720 1.20.120.1300
3f46
The crystal structure of c176a mutated [fe]-hydrogenase (hmd) holoenzyme from methanocaldococcus jannaschii
Source: Methanocaldococcus jannaschii. Methanococcus jannaschii. Organism_taxid: 2190. Strain: dsm 2661. Gene: hmd, mj0784. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (345 residues) CATH domains: 3.40.50.720 1.20.120.1300
3f47
The crystal structure of [fe]-hydrogenase (hmd) holoenzyme from methanocaldococcus jannaschii
Source: Methanocaldococcus jannaschii. Methanococcus jannaschii. Organism_taxid: 2190. Strain: dsm 2661. Gene: hmd, mj0784. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (344 residues) CATH domains: 3.40.50.720 1.20.120.1300
3h65
The crystal structure of c176a mutated [fe]-hydrogenase (hmd) holoenzyme in complex with methylenetetrahydromethanopterin
Source: Methanocaldococcus jannaschii. Methanococcus jannaschii. Organism_taxid: 2190. Strain: dsm 2661. Gene: hmd, mj0784. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (345 residues) CATH domains: 3.40.50.720 1.20.120.1300
Bound ligand:   Het Group H4M corresponds to enzyme reactant 5,10-methenyl-5,6,7,8-tetrahydromethanopterin
4jjf
Crystal structure of fe-hydrogenase from methanothermobacter marburgensis in complex with 2-naphthylisocyanide
Source: Methanothermobacter marburgensis. Organism_taxid: 79929. Strain: dsm 2133 / 14651 / nbrc 100331 / ocm 82 / marburg
Chains: A, B (344 residues) CATH domains: 3.40.50.720 1.20.120.1300
4jjg
Crystal structure of fe-hydrogenase from methanothermobacter marburgensis in complex with toluenesulfonylmethylisocyanide
Source: Methanothermobacter marburgensis. Organism_taxid: 79929. Strain: dsm 2133 / 14651 / nbrc 100331 / ocm 82 / marburg
Chains: A, B (344 residues) CATH domains: 3.40.50.720 1.20.120.1300
5ok4
Crystal structure of native [fe]-hydrogenase hmd from methanothermobacter marburgensis inactivated by o2.
Source: Methanothermobacter marburgensis str. Marburg. Organism_taxid: 79929. Cell_line: /. Organ: /. Tissue: /
Chain: A (344 residues)
6ggu
Crystal structure of native fe-hydrogenase from methanothermobacter marburgensis
Source: Methanothermobacter marburgensis str. Marburg. Methanobacterium thermoautotrophicum. Organism_taxid: 79929. Variant: /. Cell_line: /. Atcc: /. Organ: /. Tissue: /
Chain: A (344 residues)
6hac
Crystal structure of [fe]-hydrogenase (hmd) holoenzyme from methanococcus aeolicus (open form)
Source: Methanococcus aeolicus (strain atcc baa-1280 / dsm 17508 / ocm 812 / nankai-3). Organism_taxid: 419665. Variant: /. Cell_line: /. Atcc: /. Organ: /. Tissue: /. Cell: /.
Chain: A (342 residues)
6hae
Crystal structure of [fe]-hydrogenase (hmd) from methanococcus aeolicus in complex with fegp cofactor and methenyl- tetrahydromethanopterin (close form b)
Source: Methanococcus aeolicus (strain atcc baa-1280 / dsm 17508 / ocm 812 / nankai-3). Organism_taxid: 419665. Strain: atcc baa-1280 / dsm 17508 / ocm 812 / nankai-3. Variant: /. Cell_line: /. Atcc: /. Organ: /. Tissue: /.
Chains: A, K (342 residues)
Bound ligand:   Het Group E4M corresponds to enzyme reactant 5,10-methenyl-5,6,7,8-tetrahydromethanopterin
6hav
Crystal structure of [fe]-hydrogenase (hmd) from methanococcus aeolicus in complex with fegp and methenyl-tetrahydromethanopterin (close form a) at 1.06 a resolution
Source: Methanococcus aeolicus (strain atcc baa-1280 / dsm 17508 / ocm 812 / nankai-3). Organism_taxid: 419665. Variant: /. Cell_line: /. Atcc: /. Organ: /. Tissue: /. Cell: /.
Chain: A (342 residues)
Bound ligand:   Het Group E4M corresponds to enzyme reactant 5,10-methenyl-5,6,7,8-tetrahydromethanopterin
6hux
Hmdii from methanocaldococcus jannaschii reconstitued with fe- guanylylpyridinol (fegp) cofactor and co-crystallized with methenyl- tetrahydromethanopterin at 2.5 a resolution
Source: Methanocaldococcus jannaschii dsm 2661. Methanococcus jannaschii. Organism_taxid: 243232. Cell_line: /. Atcc: /. Organ: /. Tissue: /. Cell: /. Gene: mj1338.
Chain: A (369 residues)
Bound ligand:   Het Group E4M is 55.56% similar to enzyme reactant 5,10-methenyl-5,6,7,8-tetrahydromethanopterin
6huy
Hmdii from desulfurobacterium thermolithotrophum reconstitued with fe- guanylylpyridinol (fegp) cofactor and co-crystallized with methenyl- tetrahydrofolate form a
Source: Desulfurobacterium thermolithotrophum dsm 11699. Organism_taxid: 868864. Cell_line: /. Atcc: /. Organ: /. Tissue: /. Cell: /. Gene: dester_1504.
Chains: A, C (350 residues)
6yk9
[Fe]-hydrogenase from methanolacinia paynteri with bound guanylylpyridinol at 1.7-a resolution
Source: Methanolacinia paynteri g-2000. Organism_taxid: 694436. Variant: dsm 2545. Cell_line: /. Atcc: /. Organ: /. Tissue: /. Cell: /. Gene: hmd.
Chains: A, B, C, E (341 residues)
6yka
Asymmetric [fe]-hydrogenase from methanolacinia paynteri apo and in complex with fegp at 2.1-a resolution
Source: Methanolacinia paynteri g-2000. Organism_taxid: 694436. Cell_line: /. Atcc: /. Organ: /. Tissue: /. Cell: /. Gene: hmd. Expressed in: escherichia coli bl21(de3).
Chains: A, B (341 residues)
6ykb
[Fe]-hydrogenase from methanolacinia paynteri in complex with gmp at 1.55-a resolution
Source: Methanolacinia paynteri g-2000. Organism_taxid: 694436. Variant: dsm 2545. Cell_line: /. Atcc: /. Organ: /. Tissue: /. Cell: /. Gene: hmd.
Chains: A, C (341 residues)