The enzyme catalyzes the protein ubiquitination. It uses a magnesium ion in two distinct steps of the reaction to bind and activate ATP PMID20797627.
DETAILS ON METAL
Not available |
|
| The magnesium ions are directly involved in ATP binding and activation |
| Site type | Physiological Metal |
| mononuclear | magnesium |
| Metal in the Resting State | |||
| Oxidation State |
Coordination Geometry | Coordination Number | PDB |
| 2 | unknownGeometry | unknown | - |
| Metal in the reaction stages | ||||||
| Step | Metal function(s) | Initial-Final Oxid. states | Initial-Final Geometry | Initial-Final Coord. # | Initial PDB | Final PDB |
| Step 1 | Coordinates substrate Increases electrophilicity Electrostatic stabiliser |
+2 - +2 |
octahedral - octahedral |
6 - 6 | - | - |
| Step 2 | Not present |
+2 - +2 |
unknownGeometry - unknownGeometry |
unknown - unknown | - | - |
| Step 3 | Not present |
+2 - +2 |
unknownGeometry - unknownGeometry |
unknown - unknown | - | - |
| Step 4 | Coordinates substrate Increases electrophilicity Electrostatic stabiliser |
+2 - +2 |
octahedral - octahedral |
unknown - unknown | - | - |
| Step 5 | Not present |
+2 - +2 |
unknownGeometry - unknownGeometry |
unknown - unknown | - | - |
| Step 6 | Not present |
+2 - +2 |
unknownGeometry - unknownGeometry |
unknown - unknown | - | - |
| Step 7 | Not present |
+2 - +2 |
unknownGeometry - unknownGeometry |
unknown - unknown | - | - |
| Step 8 | Not present |
+2 - +2 |
unknownGeometry - unknownGeometry |
unknown - unknown | - | - |
* N.B. There may be steps that are not described in between two annotated steps. Consequently the product species of step n may differ from the reagent species of step n+1.