The enzyme catalyzes the assembly of asparagine from aspartate, Mg-ATP, and glutamine PMID10587437.
DETAILS ON METAL
Not available |
|
| The magnesium ion is directly involved in the catalytic reaction (ATP binding and activation) |
| Site type | Physiological Metal |
| mononuclear | magnesium |
| Metal in the Resting State | |||
| Oxidation State |
Coordination Geometry | Coordination Number | PDB |
| 2 | unknownGeometry | unknown | - |
| Metal in the reaction stages | ||||||
| Step | Metal function(s) | Initial-Final Oxid. states | Initial-Final Geometry | Initial-Final Coord. # | Initial PDB | Final PDB |
| Step 1 | Not present |
+2 - +2 |
unknownGeometry - unknownGeometry |
unknown - unknown | - | - |
| Step 2 | Not present |
+2 - +2 |
unknownGeometry - unknownGeometry |
unknown - unknown | - | - |
| Step 3 | Not present |
+2 - +2 |
unknownGeometry - unknownGeometry |
unknown - unknown | - | - |
| Step 4 | Not present |
+2 - +2 |
unknownGeometry - unknownGeometry |
unknown - unknown | - | - |
| Step 5 | Coordinates substrate Increases electrophilicity Electrostatic stabiliser |
+2 - +2 |
octahedral - octahedral |
6 - 6 | - | - |
| Step 6 | Coordinates substrate Electrostatic stabiliser |
+2 - +2 |
octahedral - octahedral |
6 - 6 | - | - |
| Step 7 | Unknown |
+2 - +2 |
unknownGeometry - unknownGeometry |
unknown - unknown | - | - |
| Step 8 | Unknown |
+2 - +2 |
unknownGeometry - unknownGeometry |
unknown - unknown | - | - |
* N.B. There may be steps that are not described in between two annotated steps. Consequently the product species of step n may differ from the reagent species of step n+1.









