The enzyme catalyzes the cyclization of farnesyl diphosphate to form epi-isozizaene. It binds three magnesium ions which directly partecipates to the reaction mechanism (PMID20131801).
CATALYTIC METAL(S)
| Metal/Site Information | |||||
| Residue in MACiE* | Atom in MACiE* | Residue Type in MACiE | Physiological Metal |
Site Type |
Function(s) |
| MG 700 A | MG | magnesium | magnesium | trinuclear | Coordinates substrate Promotes heterolysis Electrostatic stabiliser |
| MG 701 A | MG | magnesium | magnesium | trinuclear | Coordinates substrate Promotes heterolysis Electrostatic stabiliser |
| MG 702 A | MG | magnesium | magnesium | trinuclear | Coordinates substrate Promotes heterolysis Electrostatic stabiliser |
*It refers to the MACiE reference pdb: 3kb9
| Metal/s Properties in Resting State | ||||||
| MG 700 A | Resting state enzyme (-) | |||||
| Oxidation State | 2 | |||||
| Coordination Geometry | unknownGeometry | |||||
| Coordination Number | unknown | |||||
| Notes | - | |||||
| MG 701 A | Resting state enzyme (-) | |||||
| Oxidation State | 2 | |||||
| Coordination Geometry | unknownGeometry | |||||
| Coordination Number | unknown | |||||
| Notes | - | |||||
| MG 702 A | Resting state enzyme (-) | |||||
| Oxidation State | 2 | |||||
| Coordination Geometry | unknownGeometry | |||||
| Coordination Number | unknown | |||||
| Notes | - | |||||
| References |
| -Lin X, Cane DE, Christianson DW Structure of epi-isozizaene synthase from Streptomyces coelicolor A3(2), a platform for new terpenoid cyclization templates. Biochemistry. 2010 Mar 2;49(8):1787-97.(MEDLINE:20131801) |