The enzyme catalyses the oxidative coupling of a variety of aromatic phenols by using the oxidant H2O2 as a cofactor. The enzyme binds two structural calcium ions and a catalytic Fe-heme cofactor PMID12668462.
CATALYTIC METAL(S)
| Metal/Site Information | |||||
| Residue in MACiE* | Atom in MACiE* | Residue Type in MACiE | Physiological Metal |
Site Type |
Function(s) |
| CA 501 A | CA | calcium | calcium | mononuclear | Structural (in the active site) |
| CA 502 A | CA | calcium | calcium | mononuclear | Structural (in the active site) |
| HEM 350 A | FE | iron | iron | haem | Coordinates substrate One electron donor Radical stabiliser One electron acceptor |
*It refers to the MACiE reference pdb: 7atj
| Metal/s Properties in Resting State | ||||||
| CA 501 A | Resting state enzyme (1arp) | |||||
| Oxidation State | 2 | |||||
| Coordination Geometry | pentagonalBipyramidal | |||||
| Coordination Number | 7 | |||||
| Notes | - | |||||
| CA 502 A | Resting state enzyme (1arp) | |||||
| Oxidation State | 2 | |||||
| Coordination Geometry | pentagonalBipyramidal | |||||
| Coordination Number | 8 | |||||
| Notes | - | |||||
| HEM 350 A | Resting state enzyme (1arp) | |||||
| Oxidation State | 3 | |||||
| Coordination Geometry | octahedral | |||||
| Coordination Number | 6 | |||||
| Notes | - | |||||
| References |
| -Laberge M, Huang Q, Schweitzer-Stenner R, Fidy J The endogenous calcium ions of horseradish peroxidase C are required to maintain the functional nonplanarity of the heme. Biophys J. 2003 Apr;84(4):2542-52.(MEDLINE:12668462) |
| -Derat E, Shaik S An efficient proton-coupled electron-transfer process during oxidation of ferulic acid by horseradish peroxidase: coming full cycle. J Am Chem Soc. 2006 Oct 25;128(42):13940-9.(MEDLINE:17044722) |