The enzyme catalyzes the Ca2+-dependent hydrolysis of both DNA and RNA at the 5 position of the phosphodiester bond yielding a free 5-hydroxyl group and a 3-phosphate monoester (PMID288045).
DETAILS ON METAL
CA 1 x |
|
| The enzyme binds a calcium ion which is directly involved in the catalytic mechanism (substrate binding and activation and charge stabilization) (PMID288045). |
| Site type | Physiological Metal |
| mononuclear | calcium |
| Metal in the Resting State | |||
| Oxidation State |
Coordination Geometry | Coordination Number | PDB |
| 2 | unknownGeometry | 5 | 1ena |
| Metal in the reaction stages | ||||||
| Step | Metal function(s) | Initial-Final Oxid. states | Initial-Final Geometry | Initial-Final Coord. # | Initial PDB | Final PDB |
| Step 1 | Coordinates substrate Increases electrophilicity Electrostatic stabiliser |
+2 - +2 |
unknownGeometry - unknownGeometry |
4 - unknown | 2sns | - |
| Step 2 | Not active |
+2 - +2 |
unknownGeometry - unknownGeometry |
5 - 5 | 1ena | 1ena |
* N.B. There may be steps that are not described in between two annotated steps. Consequently the product species of step n may differ from the reagent species of step n+1.