The enzyme catalyzes the hydrolysis of cerebroside 3-sulfate producing cerebroside and sulfate. It uses a divalent cation to perform the catalytic mechanism (PMID11124905).
MG 44 x IN THE RESTING STATE ENZYME
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Representative PDB structure for MG 44 x in the resting state enzyme | |
| PDB code | 1e1z | |
| Metal Type in the PDB | magnesium | |
| Residue name of the metal in the PDB | MG | |
| Residue number of the metal in the PDB | 700 | |
| Chain of the metal in the PDB | P | |
| Atom name of the metal in the PDB | MG | |
| Download the coordinates file of the metal site | NOTES ON PDB: In this structure the cysteine (a ligand of the metal ion) is mutated in serine. | |
| First Coordination Sphere | ||||||
| Ligand Type | Ligand Identity | Residue In MACiE | Donors | Bind Mode | Catalytic? | Notes |
| residue | ASP 29 P | ASP 29 _ | OD1 | monodentate | No | - |
| residue | ASP 30 P | ASP 30 _ | OD1 | monodentate | No | - |
| residue | ASP 281 P | ASP 281 _ | OD1,OD2 | bidentate | Yes | Asp281 is involved proton relay. |
| residue | ASN 282 P | ASN 282 _ | OD1 | monodentate | No | - |